Navigation Links
A second pathway for antidepressants
Date:2/7/2011

itous of these transmembrane proteins, gating the passage of potassium ions through neural membranes, which sets the excitability of the neuron. Earlier studies had shown that when the TREK1 gene is "knocked out" of mice, the mice display a depression-resistant phenotype that mimics the behavior of mice treated with fluoxetine and that the antidepressant inhibits the activity of the TREK1 channel. While these results pointed to a possible role for the TREK1 ion channel in the beneficial response to fluoxetine, the mechanism behind this activity was unclear.

"Studying what the different protein parts of an ion channel do is a huge challenge," Isacoff says. "Over the years, my group has developed techniques by which the domains of channel proteins can be labeled with site-specific fluorescent dyes. Structural rearrangements of the labeled sites in the channel can then be detected through changes in the fluorescence."

Isacoff and his group separated the C-terminal domain from the rest of the protein and tagged it with a green fluorescent protein (GFP) - a fluorescent protein from jellyfish commonly used to paint cells green for biological studies. Whereas the pore of the TREK1 ion channel is embedded in the plasma membrane of a neuron, the C-terminal is a short tail that protrudes out into the surrounding cytoplasm.

Using voltage clamps to measure electrical currents through the channel and fluorescence to monitor the disposition of the C-terminal domain, Isacoff and his group found that when the C-terminal tail is fully bound to the plasma membrane, the TREK1 potassium channel opens more; when the tail is unbound from the plasma membrane, the ion channel tends to close.

"We found that fluoxetine causes the isolated C-terminal domain to unbind from the membrane and also causes an inhibition of current from the full TREK1 channel," Isacoff says.

The next step will be to see how the C-terminal tail is affected by the pre
'/>"/>

Contact: Lynn Yarris
lcyarris@lbl.gov
510-486-5375
DOE/Lawrence Berkeley National Laboratory
Source:Eurekalert  

Page: 1 2 3

Related biology news :

1. Out of mind in a matter of seconds
2. MegaMatcher Accelerator 3.0 for Rapid Biometric Identification Matches Up to 200 Million Irises or 100 Million Fingerprints Per Second
3. OHSU Doernbecher Childrens Hospital conducts second phase of landmark Batten study
4. Probing the mysterious second-wave of damage in head injury patients
5. Second-generation device more effective in capturing circulating tumor cells
6. Autism Speaks awards second class of Dennis Weatherstone pre-doctoral fellowships
7. Aware, Inc. Reports Second Quarter 2010 Financial Results
8. BIO-key® Announces Second Quarter 2010 Earnings Release and Conference Call Schedule
9. Teleconference Alert: LyondellBasell Reviews Second Quarter 2010 Financial Results
10. BIO-key® Reports Strong 2010 Second Quarter and Six Month Financial Results
11. De La Rue Provides Second-Generation ePassport for Malta
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
A second pathway for antidepressants
(Date:7/25/2014)... WA Nearly 600 scientists from 25 countries ... Genetics Meeting organized by the Genetics Society of ... Washington in Seattle. The conference will feature close ... research results on topics including gene expression and ... emerging technologies, evolution, aging, and a variety of ...
(Date:7/25/2014)... congenital central nervous system disease that is caused ... during the embryonic phase. Many patients have varying ... neurocognitive retardation. Such problems decrease the patients, functional ... Ankara Physical Medicine and Rehabilitation Education and Research ... with SB, using the Pediatric Evaluation of Disability ...
(Date:7/25/2014)... the first time, Spanish researchers have detected an unknown ... cells are introduced into a droplet of salt ... sodium chloride crystallisation to create biomineralogical biosaline 3D morphologically ... the material, bacteria are revived. The discovery was made ... the cover of the Astrobiology journal and ...
Breaking Biology News(10 mins):Yeast meeting to showcase latest breakthroughs in genetics and molecular biology 2Bacteria manipulate salt to build shelters to hibernate 2
... of the body, the great majority of genes are silenced, ... up chromosomes. Estimates are that only about 10 percent of ... or "on," at any given time in a particular cell ... , Reliable gene silencing is vital to the health of ...
... have discovered a gene in fruit flies that helps ... The study, published in the April 4 issue of ... in mammals. , In teasing apart the molecular interactions ... gene they dubbed "Lazaro" that is expressed 15 times ...
... industry, including cruise lines, local governments and shore ... are taking proactive measures to ensure a sustainable ... according to a new report titled From Ship ... by Conservation International's (CI) Center for Environmental Leadership ...
Cached Biology News:New mechanism for essential genome-wide gene silencing identified 2New gene reduces retinal degeneration in fruit flies 2Report finds cruise industry is protecting the precious places it visits 2Report finds cruise industry is protecting the precious places it visits 3Report finds cruise industry is protecting the precious places it visits 4
(Date:7/25/2014)... Pittsburgh, PA (PRWEB) July 25, 2014 ... annual Call for Topics for Conferee Networking. These two-hour ... similar interests to meet and resolve problems, discuss new ... , The deadline to submit a topic for consideration ... at pittcon.org . The Conferee Networking committee will ...
(Date:7/25/2014)... Israel , July 25, 2014  Galmed Pharmaceuticals ... biopharmaceutical company focused on the development and commercialization of ... diseases and cholesterol gallstones, today announced financial results for ... Half 2014 Financial Summary: , Reported cash and ... compared with $137,000 at December 31, 2013.  , ...
(Date:7/25/2014)... (PRWEB) July 25, 2014 At the ... has granted a 60-day extension for the public comment ... Special Rule for Model Aircraft established by Congress as ... 2012. The 60-day extension establishes the new deadline for ... States Department of Transportation/FAA notice published in the Federal ...
(Date:7/24/2014)... 2014 -- Keryx Biopharmaceuticals, Inc. (Nasdaq:KERX) (the "Company") announced ... control Phase 3 study of Zerenex (ferric citrate), the ... treatment of hyperphosphatemia in patients with end-stage renal disease ... iRon delivery with FErric CiTrate in EsrD) was published ... Society of Nephrology ( JASN ). ...
Breaking Biology Technology:Pittcon 2015 Announces Call for Topics for Conferee Networking Sessions 2Galmed Pharmaceuticals Reports First Half 2014 Financial Results 2Galmed Pharmaceuticals Reports First Half 2014 Financial Results 3Galmed Pharmaceuticals Reports First Half 2014 Financial Results 4Galmed Pharmaceuticals Reports First Half 2014 Financial Results 5Galmed Pharmaceuticals Reports First Half 2014 Financial Results 6FAA Grants Comment Extension at the Request of AMA 2Zerenex (ferric citrate) long-term Phase 3 study results published in JASN 2Zerenex (ferric citrate) long-term Phase 3 study results published in JASN 3Zerenex (ferric citrate) long-term Phase 3 study results published in JASN 4
... - In a value-added move aimed at media-savvy ... social computing craze, Sonic Foundry has ... its Mediasite web communications product. , ,The ... a Madison-based rich media company, calls Mediasite Podcast. ...
... for the next wave of high-growth investments, and judging by ... range of opportunities await later-stage institutional investors. , ,In its ... Venture Research at the University of New Hampshire ... in 2006, with total investments of $25.6 billion. , ...
... yet to turn a profit, and it operates in a biotech ... by Madison research tool manufacturer NimbleGen Systems to file ... the latest bit of evidence that the Wisconsin biotech company is ... of growth and visibility for NimbleGen, which saw its revenues grow ...
Cached Biology Technology:National angel investment grew 11 percent in `06 2National angel investment grew 11 percent in `06 3IPO-bound NimbleGen traveling in fast company 2IPO-bound NimbleGen traveling in fast company 3IPO-bound NimbleGen traveling in fast company 4
...
Goat polyclonal to Rabenosyn 5 ( Abpromise for all tested applications). Antigen: Synthetic peptide: ELKHTLAKQKGGTD, corresponding to C terminal amino acids 771-784 of Human Rabenosyn 5. Entr...
... ( Abpromise for all tested ... Synthetic peptide derived from residues 231 ... (Note: the amino acid sequence is ... Entrez Gene ID: 80829 ...
Anti-Salmonid Ig (H), Alk Phos, (Clone IPA5F12) (mouse IgG2a)...
Biology Products: