Navigation Links
A radar for ADAR: Altered gene tracks RNA editing in neurons
Date:12/25/2011

requiring ADAR to fix a purposely broken individual letter of RNA on an engineered gene.

"We're actually repairing RNA at the level of a single informational bit, or nucleotide," Reenan said. "Here we've shown we can take a mutant version of a gene and restore its function, but at the level of RNA rather than DNA."

A reporter of an editor

Reenan and third author Kyle Jay began working to create the reporter in 2006 when Jay was an undergraduate student just embarking on what would become a celebrated senior thesis at Brown. They started with a well-known tool of molecular biology: a jellyfish gene that produces a protein that glows green upon exposure to ultraviolet light. The strategy was to intentionally break the gene in a way that ADAR is uniquely suited to fix.

First they engineered the gene to include necessary "intron" code that requires a specific splicing operation to take place. Then they inserted the "stop codon" T-A-G in place of T-G-G, which causes transcription to cease, effectively preventing production of the green fluorescent protein. But before splicing occurs and when ADAR finds the stop codon U-A-G in the RNA transcript, it edits the A to an I, which restores the correct information, and translation of the whole gene proceeds as if there were no stop mutation in the DNA. So when splicing and ADAR editing occurs, neurons with the gene reporter glow green.

To see where ADAR editing and splicing were occurring, compared to just splicing alone, they also rigged up an engineered gene with the splicing requirement, but not the T-A-G codon. That would produce yellow fluorescent protein when splicing alone occurred.

Armed with their new ADAR reporter, Reenan and lead author James Jepson set out to make some biological observations in flies. One was that ADAR activity is more pronounced in certain parts of the brains of developing larvae than it is in the brains of adults. The team also found w
'/>"/>

Contact: David Orenstein
david_orenstein@brown.edu
401-863-1862
Brown University
Source:Eurekalert  

Page: 1 2 3

Related biology news :

1. TEEB report puts worlds natural assets on the global political radar
2. New boreal forest biomass maps produced from radar satellite data
3. SRI International announces findings from new upper atmospheric radar system for scientific research
4. A glimpse of future GMES Sentinel-1 radar images
5. New pollution radar developed to provide unprecedented picture of urban smog
6. Researcher finds altered cerebella in those with Down syndrome
7. Clinical study suggests estrogen levels and breast health can be altered
8. Altered gene protects some African-Americans from coronary artery disease
9. Most river flows across the US are altered by land and water management
10. 1st census shows life in planet ocean is richer, more connected, more altered than expected
11. New data on the regulation of a protein that is altered in all cancers
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
A radar for ADAR: Altered gene tracks RNA editing in neurons
(Date:12/17/2014)... --Research and Markets ( http://www.researchandmarkets.com/research/s6qzt5/samsung_galaxy_s5 ) ... Galaxy S5 - Home Button Synaptics Fingerprint Sensor ... http://photos.prnewswire.com/prnh/20130307/600769 ... the iPhone 5S, Samsung introduces for the first ... The Galaxy S5 home button presents an ...
(Date:12/17/2014)... Research and Markets ( http://www.researchandmarkets.com/research/9zq33j/global_chemical ... "Global Chemical Sensor Market 2015-2019" report to ... One major trend upcoming in ... sensors in biomedical applications. Chemical sensors help in ... diagnosis during surgical procedures. The Global Chemical Sensor ...
(Date:12/11/2014)... 09, 2014 Research and Markets ... ( http://www.researchandmarkets.com/research/9ql3kr/biometrics_market ) has announced the addition ... report to their offering. ... the adoption of multimodal biometric systems. Multimodal biometric ... individual for verification and identification purposes. This helps ...
Breaking Biology News(10 mins):Samsung Galaxy S5 - Home Button Synaptics Fingerprint Sensor Technology Analysis 2Global Chemical Sensor Market 2015-2019: Key Vendors are Abbott Laboratories, Bayer, Hoffmann La-Roche, Johnson & Johnson, NGK Spark Plugs and Robert Bosch 2Biometrics Market in India 2015-2019: Key Vendors are 3M Cogent, NEC, Safran and Suprema 2
... Based on a pilot study in children with sarcoma, ... that immunotherapy could prove beneficial in treating high-risk forms ... novel dendritic vaccine as well as a standard flu ... Their findings, published in the August 1 issue ...
... surprisingly simple, inexpensive technique, chemists have found a way ... amounts of electricity, common chemicals and a room-temperature glass ... fuels, many researchers have proposed using solar electricity to ... when the sun goes down. One of the chief ...
... NY In the United States, Lyme disease is ... from animals to humans. These animal-borne diseases can make ... and effective intervention are crucial to protecting the public. ... Millbrook, NY recently received $750,000 in grant funding from ...
Cached Biology News:Immunotherapy in high-risk pediatric sarcomas shows promising response 2Immunotherapy in high-risk pediatric sarcomas shows promising response 3Water refineries? 2Water refineries? 3EPA funds ground-breaking Lyme disease research 2
(Date:12/24/2014)... SPRING, Md. , Dec. 24, 2014 /PRNewswire/ ... announced today that Medtronic, Inc. (NYSE: ... to the U.S. Food and Drug Administration (FDA) ... II implantable drug infusion system (including a newly ... ® (treprostinil) Injection delivered intravenously to patients ...
(Date:12/24/2014)... , Dec. 24, 2014  Vermillion, Inc. (Nasdaq: ... gynecologic disease, announced today that the Company closed ... Oracle Investment Management, Jack W. Schuler , ... proceeds were $10.5 million, before offering expenses.  The ... general corporate purposes. Under the terms ...
(Date:12/24/2014)... “Preparative & Process Chromatography Market by Instrument ... Buffers, Valves, Guages, Seals), Accessories, Services, End User ... 2019” provides a detailed overview of the major ... strategies impacting the preparative and process chromatography market ... revenue and share analysis. , Full Copy ...
(Date:12/24/2014)... 23, 2014 Earlier this year in ... Sherley, director of the Adult Stem Cell Technology Center, ... overlooked and under appreciated unique property of adult tissue ... Stem Cells: Misunderstood in the Past, Important for the ... participants. He gave the address at the 4th ...
Breaking Biology Technology:United Therapeutics Announces Submission Of Pre-Market Approval Application For Implantable Drug Infusion System To Deliver Remodulin 2United Therapeutics Announces Submission Of Pre-Market Approval Application For Implantable Drug Infusion System To Deliver Remodulin 3United Therapeutics Announces Submission Of Pre-Market Approval Application For Implantable Drug Infusion System To Deliver Remodulin 4Vermillion Announces Closing of Equity Financing 2Vermillion Announces Closing of Equity Financing 3Preparative and Process Chromatography Market is expected to reach $9 billion by 2019 - New Report by Marketsandmarkets 2Preparative and Process Chromatography Market is expected to reach $9 billion by 2019 - New Report by Marketsandmarkets 3Preparative and Process Chromatography Market is expected to reach $9 billion by 2019 - New Report by Marketsandmarkets 4Adult Stem Cell Technology Center, LLC’s Director Sherley's Address on What’s Holding Back Regenerative Medicine Featured on Omics Group Website 2Adult Stem Cell Technology Center, LLC’s Director Sherley's Address on What’s Holding Back Regenerative Medicine Featured on Omics Group Website 3Adult Stem Cell Technology Center, LLC’s Director Sherley's Address on What’s Holding Back Regenerative Medicine Featured on Omics Group Website 4
... that it has signed an asset purchase agreement to ... Bulletin,Board: IMMC) for $31 million in cash subject to ... certain claims owing to,Veridex, LLC and the assumption of ... include intellectual property, product,inventory and clinical data as well ...
... announced the launch,of the WellCentive Secure Messaging ... its,popular online patient registry system for health ... ) provides a web-based,preventive care and chronic ... health insurance companies and other,health care organizations. ...
... leading,provider of screening solutions for drug discovery, today ... agreement Cellectricon,will provide AstraZeneca with two of its ... screening., Cellectricon and AstraZeneca formed and announced ... solution to major bottlenecks in ligand gated ion,channel ...
Cached Biology Technology:Veridex Announces Agreement to Acquire the Assets of Immunicon Corporation 2WellCentive Launches New Secure Messaging Platform for Its Patient Registry System 2WellCentive Launches New Secure Messaging Platform for Its Patient Registry System 3Cellectricon and AstraZeneca - Partners in New High Throughput Ion Channel Drug Discovery Technology 2
... Invitrogen makes blotting of E-PAGE Gels ... pre-cut fiber blotting pad and several types ... perfectly fit 8.6 cm x 13.5 cm ... PVDF membranes are designed for western transfer ...
... intended for laboratory research use only. ... buffered formalin and embedded in paraffin. ... certified pathologists. Tissue cores were removed ... a new recipient paraffin block, in an ...
... CAST Equine EIA Principle: ... LTD4 and LTE4 are products ... 5-lipoxygenase and glutathione-S-transferase. In the ... "Slow Reacting Substances of Anaphylaxis ...
... the basic components and protocol required ... plates for quantitation of a specific ... culture supernate. Every Screening Set contains ... (5 individual standards), Streptavidin-HRP, TMB Substrate ...
Biology Products: