Navigation Links
A radar for ADAR: Altered gene tracks RNA editing in neurons
Date:12/25/2011

requiring ADAR to fix a purposely broken individual letter of RNA on an engineered gene.

"We're actually repairing RNA at the level of a single informational bit, or nucleotide," Reenan said. "Here we've shown we can take a mutant version of a gene and restore its function, but at the level of RNA rather than DNA."

A reporter of an editor

Reenan and third author Kyle Jay began working to create the reporter in 2006 when Jay was an undergraduate student just embarking on what would become a celebrated senior thesis at Brown. They started with a well-known tool of molecular biology: a jellyfish gene that produces a protein that glows green upon exposure to ultraviolet light. The strategy was to intentionally break the gene in a way that ADAR is uniquely suited to fix.

First they engineered the gene to include necessary "intron" code that requires a specific splicing operation to take place. Then they inserted the "stop codon" T-A-G in place of T-G-G, which causes transcription to cease, effectively preventing production of the green fluorescent protein. But before splicing occurs and when ADAR finds the stop codon U-A-G in the RNA transcript, it edits the A to an I, which restores the correct information, and translation of the whole gene proceeds as if there were no stop mutation in the DNA. So when splicing and ADAR editing occurs, neurons with the gene reporter glow green.

To see where ADAR editing and splicing were occurring, compared to just splicing alone, they also rigged up an engineered gene with the splicing requirement, but not the T-A-G codon. That would produce yellow fluorescent protein when splicing alone occurred.

Armed with their new ADAR reporter, Reenan and lead author James Jepson set out to make some biological observations in flies. One was that ADAR activity is more pronounced in certain parts of the brains of developing larvae than it is in the brains of adults. The team also found w
'/>"/>

Contact: David Orenstein
david_orenstein@brown.edu
401-863-1862
Brown University
Source:Eurekalert  

0

GOOD

Page: 1 2 3

Related biology news :

1. TEEB report puts worlds natural assets on the global political radar
2. New boreal forest biomass maps produced from radar satellite data
3. SRI International announces findings from new upper atmospheric radar system for scientific research
4. A glimpse of future GMES Sentinel-1 radar images
5. New pollution radar developed to provide unprecedented picture of urban smog
6. Researcher finds altered cerebella in those with Down syndrome
7. Clinical study suggests estrogen levels and breast health can be altered
8. Altered gene protects some African-Americans from coronary artery disease
9. Most river flows across the US are altered by land and water management
10. 1st census shows life in planet ocean is richer, more connected, more altered than expected
11. New data on the regulation of a protein that is altered in all cancers
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
A radar for ADAR: Altered gene tracks RNA editing in neurons
(Date:5/22/2013)... have developed a novel technique that can detect molecular ... that is one of the most important, though time-consuming, ... a paper in Nature , post-doctoral researcher David ... Schnell of the Center for Free-Electron Laser Science (CFEL) ... finely-tuned microwave fields to identify molecular variants apart, and ...
(Date:5/22/2013)... and human health effects from disposal of millions ... scientists to recommend stronger government policies to encourage ... materials. That,s the conclusion of a new paper ... Technology . , Oladele A. Ogunseitan and colleagues ... for powering everything from smart phones to components ...
(Date:5/22/2013)... May 22, 2013 Early screening for prostate cancer ... testing is for women, thanks to UC Irvine research ... Chemical Society . , After more than a decade ... to clearly identify clinically usable markers for prostate cancer ... far sooner, with greater accuracy and at dramatically lower ...
Breaking Biology News(10 mins):Detecting mirror molecules 2Detecting mirror molecules 3UCI chemists devise inexpensive, accurate way to detect prostate cancer 2UCI chemists devise inexpensive, accurate way to detect prostate cancer 3
... University of Minnesota,s Water Resources Center has authored a ... lakes, rivers and groundwater for the 21st century and ... Minnesota House of Representative,s Environment, Energy and Natural Resources ... Jan. 5 in Room 5 of the State Office ...
... Unisys Corporation (NYSE: UIS ... next year, Unisys predicts, six disruptive technology trends that are ... in the enterprise as organizations seek practical ways to make ... secure and cost-efficient. "Practicality will be the theme ...
... January 4, 2011 Researchers from The Scripps ... produced a long-lasting anti-cocaine immunity in mice by giving them ... virus with a particle that mimics cocaine. In their ... of Molecular Therapy , the researchers say this novel ...
Cached Biology News:U of Minnesota center releases nation's first long-term framework for statewide water sustainability 2From Disruptive to Productive: Unisys Predicts 2011 to be Year of Practical Application of Cutting-Edge Technologies 2From Disruptive to Productive: Unisys Predicts 2011 to be Year of Practical Application of Cutting-Edge Technologies 3From Disruptive to Productive: Unisys Predicts 2011 to be Year of Practical Application of Cutting-Edge Technologies 4From Disruptive to Productive: Unisys Predicts 2011 to be Year of Practical Application of Cutting-Edge Technologies 5Team creates novel vaccine that produces strong immunity against cocaine high 2Team creates novel vaccine that produces strong immunity against cocaine high 3Team creates novel vaccine that produces strong immunity against cocaine high 4
(Date:5/23/2013)... Ill. , May 23, 2013 Z ... providing value-added ingredients to a variety of industries, announced ... in a panel discussion at this year,s National Restaurant ... 20-21, 2013, on behalf of the Research Chefs Association.  ... for Restaurants: The Good, the Bad and the Ugly," ...
(Date:5/23/2013)... , May 23, 2013 Pressure BioSciences, Inc. ... the Company will host a teleconference to discuss its ... business update. The teleconference information is provided below: ... Eastern Daylight Time (EDT) Dial-in: (800) 875-3456 ... 607-2001 (Int,l) Passcode: VS22823 For those unable ...
(Date:5/23/2013)... , May 23, 2013 Pusan ... Inc., a private biotherapeutics and contract research company ... products for cancer, announced today a publication reporting ... treated with the oncolytic and immunotherapeutic vaccinia JX-594 ... research was published in the May 15 th ...
(Date:5/23/2013)... Bed bugs already cost Alton Housing Authority thousands. ... a report from kmov.com suggested that the said office might ... Meanwhile, to be of help, My Cleaning Products gave tips ... bug exterminator service. , The report, which was posted ... spent $35,000 for bed bug elimination, My Cleaning ...
Breaking Biology Technology:Z Trim's Chef Erin Ryan sits on Expert Panel at NRA Show 2Z Trim's Chef Erin Ryan sits on Expert Panel at NRA Show 3Pressure BioSciences, Inc. to Discuss First Quarter 2013 Financial Results and Provide Business Update 2Pusan National University Announces Science Translational Medicine Publication Highlighting Landmark Demonstration of Functional Anti-Cancer Antibody Induction in Patients Following JX-594 Treatment 2Pusan National University Announces Science Translational Medicine Publication Highlighting Landmark Demonstration of Functional Anti-Cancer Antibody Induction in Patients Following JX-594 Treatment 3Bed Bug Exterminator Service Could Cost AHA $250K, My Cleaning Products Gives Tips How to Save Apartments from Costly Bed Bug Treatment 2
... third-party plug-in for popular embedded tool suite. , ... ... Quadros Systems, Inc., a leading supplier of configurable and scalable ... module for the RTXC Quadros RTOS is now available for ...
... on Tuesday, July 15 at 4:30 p.m. Eastern -, ... VRML ), a molecular diagnostics company, will host a ... Ueland,M.D., Principal Investigator on the submission and Associate Professor ... the keynote,speaker of the roundtable., Vermillion,s ovarian tumor ...
... 11 Signalife,Inc. (Amex: SGN ) announced ... its previously-announced merger or similar business combination with,Heart ... has been,appointed to proceed with due diligence and ... presently contemplated, a new parent,company will be established ...
Cached Biology Technology:RTXC Kernel Awareness Module from Quadros Systems Now Available for Keil Vision Debugger 2RTXC Kernel Awareness Module from Quadros Systems Now Available for Keil Vision Debugger 3RTXC Kernel Awareness Module from Quadros Systems Now Available for Keil Vision Debugger 4Vermillion to Host Live Roundtable Discussion With Principal Investigator of OVA1(TM) Submission 2Vermillion to Host Live Roundtable Discussion With Principal Investigator of OVA1(TM) Submission 3Signalife Board Approves Merger 2Signalife Board Approves Merger 3
...
Human Regulatory T cell Staining Kit #2 (w/ APC Foxp3 PCH101, FITC CD4, PE CD25)...
Goat anti-VAV2 Class: Antibody Product Group: Miscellaneous Antibody...
Rabbit polyclonal to M13 Bacteriophage Coat Proteins ( Abpromise for all tested applications)....
Biology Products: