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A novel surface marker helps scientists 'fish out' mammary gland stem cells
Date:4/11/2013

del] is that by stopping GFP expression, you can directly measure the number of cell divisions that have happened since GFP was turned off," Dos Santos explains. "The cells that divide the least will carry GFP the longest and are the ones we characterized."

Using this approach, Dos Santos and her colleagues were able to select stem cells in the mammary glands to examine their gene expression signature. They confirmed that a purification method that used Cd1d, in combination with other known markers, greatly enhanced purity compared to other methods, including those previously published.

"With this advancement, we are now able to profile normal and cancer stem cells at a very high degree of purity , and perhaps point out which genes should be investigated as the next breast cancer drug targets," says Professor Hannon, who is also an Investigator of the Howard Hughes Medical Institute.


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Contact: Peter Tarr
tarr@cshl.edu
516-367-8455
Cold Spring Harbor Laboratory
Source:Eurekalert

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