Navigation Links
A new way to assemble cells into 3-D microtissues
Date:3/5/2009

a complementary repeating sequence of thymine and guanine (TGTGTG...) on the other, up to sequences whose base order varies over the full length of the 20-base strand of synthetic DNA. The more complex the sequences they display, the longer it takes the cells to bind together.

A third variable is the density of the DNA on the cell surface. By controlling how many artificial sugars the cells express, the researchers can control the DNA surface density. The greater the density, the faster the cells bind to one another.

Bertozzi says more variables for controlling cell assemblies are possible. "For example, it might be possible to cluster DNA strands on specific cellular structures. Thus, distribution of DNA on the cell surface might be yet another parameter we can exploit to guide cell-cell interactions."

Building complex microtissues one step at a time

By controlling these variables to assemble small cellular structures, then separating the desired structures from unwanted ones and unreacted cells and assembling more cells on the purified collection then repeating the steps again the researchers can synthesize large, complex microtissues in much the same way a synthetic organic chemist assembles a complex molecule.

Bertozzi and Gartner applied these methods to build a signaling network where one kind of cell controls the growth of a second kind of cell. They maintained the survival and replication of hematopoietic progenitor cells (a kind of stem cell for blood cells), which depend on the presence of the growth factor interleukin-3, by combining them in microtissues with CHO cells (Chinese hamster ovary cells) that were engineered to secrete interleukin-3.

When the two cell types were randomly mixed, the stem cells didn't grow. But structured microtissues built from the two cell types stimulated their own growth, forming a simple artificial signaling network that behaved much like the natural network
'/>"/>

Contact: Paul Preuss
paul_preuss@lbl.gov
510-486-6249
DOE/Lawrence Berkeley National Laboratory
Source:Eurekalert  

Page: 1 2 3 4

Related biology news :

1. Nanoparticles assemble by millions to encase oil drops
2. Texas Obesity Research Center at UH assembles researchers to discuss obesity
3. ESF EURYI award winner aims to stop cancer cells reading their own DNA
4. Newly created cancer stem cells could aid breast cancer research
5. AIDS interferes with stem cells in the brain
6. Clemson scientists shed light on molecules in living cells
7. Social habits of cells may hold key to fighting diseases
8. UF scientists reveal how dietary restriction cleans cells
9. Human derived stem cells can repair rat hearts damaged by heart attack
10. Scientists identify embryonic stem cells by appearance alone
11. Cells united against cancer
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
A new way to assemble cells into 3-D microtissues
(Date:10/18/2014)... In a sample of patients with undiagnosed, suspected genetic ... associated with a higher molecular diagnostic yield than traditional ... JAMA . The study is being released to ... meeting. , Exome sequencing, which sequences the protein­coding region ... genetic material present in a cell or organism), has ...
(Date:10/17/2014)... . Why do ... to reproduce? And why are there two sexes anyway? These ... of the research journal Molecular Human Reproduction published ... Bielefeld University Bielefeld has compiled this special issue on sperm ... to copulate with several males in quick succession – chimpanzees ...
(Date:10/17/2014)... available in German . ... few drugs. When treating overdoses, doctors are often limited to ... if there is a combination of drugs involved. So what ... swallows his grandmother,s pills? ETH professor Jean-Christophe Leroux from the ... an answer to this question. "The task was to develop ...
Breaking Biology News(10 mins):Study examines type of exome sequencing and molecular diagnostic yield 2Study examines type of exome sequencing and molecular diagnostic yield 3Sperm wars 2Emergency aid for overdoses 2Emergency aid for overdoses 3
... (Santa Barbara, Calif.) Viruses fill the ocean ... marine microbiology, according to a professor of biology at ... Carlson, professor with UCSB,s Department of Ecology, Evolution, and ... of marine viruses published this week by the ...
... transport system inside living cells is a well-oiled machine with ... around the cell. These molecular motors are responsible for a ... on the go. They can put themselves into "energy save ... what gets moved around the cell, and when. ...
... is available in Spanish . A ... what is causing the decline in bumble bee populations and ... the next generation of greenhouse pollinators. Bumble bees, like ... used to pollinate greenhouse crops like peppers and tomatoes. But ...
Cached Biology News:Decade-long study reveals recurring patterns of viruses in the open ocean 2Decade-long study reveals recurring patterns of viruses in the open ocean 3Carnegie Mellon scientists discover how molecular motors go into 'energy save mode' 2Carnegie Mellon scientists discover how molecular motors go into 'energy save mode' 3The flight of the bumble bee: Why are they disappearing? 2
(Date:10/20/2014)... 20, 2014 Convey Computer , ... team from Iowa State University won first place in ... a Convey HC-2ex, the team’s solution achieved the highest ... than the second place finisher. , Experts from all ... the month long challenge, using a variety of design ...
(Date:10/20/2014)... GUELPH, ON , Oct. 20, 2014 /PRNewswire/ - BIOREM Inc. ... totalling $1.7 million which brings Q3 orders to $5.8 million and ... odour control projects in North America and ... "We continue to bid new projects at record levels," said ... values were higher at the end of Q3 than they have ...
(Date:10/20/2014)... 2014  GenVec, Inc. (NASDAQ: GNVC ) today ... from its board of directors effective on October 24, 2014.  ... served as its chairman from June 2006 to November 2013.  ... Corporate Governance and Audit Committees of the board.  ... of dedicated service to GenVec, and its stockholders," said ...
(Date:10/20/2014)... OncLive® is pleased ... at Thomas Jefferson University has joined its Strategic ... Alliance Partnership program, the Sidney Kimmel Cancer Center ... awareness of the Center’s cutting-edge research programs, comprehensive ... and other health care professionals from the Sidney ...
Breaking Biology Technology:Team from Iowa State Wins 2014 MemoCODE Design Contest Using Convey HC-2ex 2Team from Iowa State Wins 2014 MemoCODE Design Contest Using Convey HC-2ex 3Zola P. Horovitz To Retire From GenVec Board 2Sidney Kimmel Cancer Center at Thomas Jefferson University Partners With OncLive 2Sidney Kimmel Cancer Center at Thomas Jefferson University Partners With OncLive 3
... Another Key Regulatory MilestoneLONDON, March 10 Monsanto,s historic ... and biotechnology has contributed to a 24 percent compound ... segment since 2001. This is one of the ... Commercial, will tell investors today as part of a ...
... has long been known for its world-record-breaking carbon ... have discovered new uses by spinning carbon nanotubes ... , Breakthroughs Without Broken Threads ... to grow carbon nanotubes of world-record breaking lengths, ...
... American Pacific Corporation (Nasdaq: APFC ) is ... Chemicals LLC (AFC), located near Sacramento California, recently inaugurated ... The new facility can accommodate either batch reactors (20 ... using up to 7 reactors from 0.25 to 1 ...
Cached Biology Technology:Monsanto's Historic R&D Investment has Contributed to Mid-20s Gross Profit Growth for Its Seeds and Genomics Segment 2Monsanto's Historic R&D Investment has Contributed to Mid-20s Gross Profit Growth for Its Seeds and Genomics Segment 3Spinning carbon nanotubes spawns new wireless applications 2AMPAC Fine Chemicals Announces the Inauguration of the New cGMP Continuous Processing Development Facility 2
... is an evolutionarily conserved form of cell ... The central component of this process ... caspases. These enzymes participate in a ... response to pro-apoptotic signals and result in ...
... is manufactured for DNA purification from agarose ... UV light. The Purification of DNA from ... DNA-cloning work. The conventional method of visualizing ... UV light damages DNA and significantly decreases ...
...
CleanBox DNA workstation with two 254nm UV lambs & 12-hr timer, 90cm (36-inch)width, single door, 115V (w/crating)...
Biology Products: