Navigation Links
A new way to assemble cells into 3-D microtissues
Date:3/5/2009

rmaceutical Chemistry at the University of California at San Francisco, based their method of assembling 3-D microtissues on an approach familiar to synthetic organic chemists, who build up novel molecular structures by combining one atom or functional group at a time. In the same way, Bertozzi and Gartner use synthetic methods to build microtissues one cell type at a time. Their results appear in the Proceedings of the National Academy of Sciences, PNAS Early Edition week of March 2, 2009.

Controlling cellular connections

The researchers enabled cells to react with other cells in a controlled way by coating the cell surfaces with DNA (not where DNA is normally found!). First they induced the cells to express artificial sugars bearing special chemical groups. Lengths of synthetic DNA, introduced into the cell-growth medium, were equipped to recognize these synthetic sugars on the cell surfaces and chemically bind to them.

The researchers coated cell surfaces from one group with strands of single-stranded DNA only 20 bases long, and the surfaces of another group with the complementary DNA strand. When a cell from one group meets its counterpart, the single strands recognize each other and form double-stranded DNA, which binds the cells together.

Bertozzi and Gartner discovered three variables that determine how cells from different groups react with one another. One is the ratio of the two kinds of cells: if both cell populations are equal, every cell finds a single partner and no complex assemblies form. But if there are, say, 50 times more cells from one group than from the other, numerous cells from the larger group will cluster around each cell from the smaller group.

Another variable is the complexity of the synthetic DNA sequence. The researchers can specify the complexity for example, from a simple repeat of two bases such as cytosine and adenine (CACACA...) on one strand, which binds to
'/>"/>

Contact: Paul Preuss
paul_preuss@lbl.gov
510-486-6249
DOE/Lawrence Berkeley National Laboratory
Source:Eurekalert  

Page: 1 2 3 4

Related biology news :

1. Nanoparticles assemble by millions to encase oil drops
2. Texas Obesity Research Center at UH assembles researchers to discuss obesity
3. ESF EURYI award winner aims to stop cancer cells reading their own DNA
4. Newly created cancer stem cells could aid breast cancer research
5. AIDS interferes with stem cells in the brain
6. Clemson scientists shed light on molecules in living cells
7. Social habits of cells may hold key to fighting diseases
8. UF scientists reveal how dietary restriction cleans cells
9. Human derived stem cells can repair rat hearts damaged by heart attack
10. Scientists identify embryonic stem cells by appearance alone
11. Cells united against cancer
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
A new way to assemble cells into 3-D microtissues
(Date:9/19/2014)... a newly discovered hadrosaur with a truly ... Rhinorex condrupus by paleontologists from North Carolina ... what is now Utah approximately 75 million years ... which translates roughly into "King Nose," was a ... hadrosaurs like Parasaurolophus and Edmontosaurus. Hadrosaurs are usually ...
(Date:9/19/2014)... of years has adapted to the arctic cold, and then ... dangerous for you. , This is happening to the small ... substance, called nonylphenol, comes from the use of certain detergents, ... endocrine disruptor, but when entering the worm it has another ... cells in its body from cold damage. , Enchytraeus ...
(Date:9/18/2014)... A sleep-promoting circuit located deep in the primitive ... Discovered by researchers at Harvard School of Medicine and ... Sciences, this is only the second "sleep node" identified ... both necessary and sufficient to produce deep sleep. , ... the study demonstrates that fully half of all of ...
Breaking Biology News(10 mins):New hadrosaur noses into spotlight 2Environmental pollutants make worms susceptible to cold 2Environmental pollutants make worms susceptible to cold 3No sedative necessary: Scientists discover new 'sleep node' in the brain 2
... it can damage blood vessels and increase the risk of ... he can thin the human blood by subjecting it to ... physics at Temple University, has pioneered the use of electric ... engines and pipelines. Now, he is using the same magnetic ...
... coastal areas in Africa can rarely utilise their entire fishing ... near the coast. Research carried out by economist Kofi Vondolia ... of fish stocks is significant to fish management. ... allows foreign fishing fleets to utilise the zones further out ...
... the Virginia Institute of Marine Science (VIMS) shows that jellyfish ... altering marine food webs by shunting food energy from fish ... frequency of jellyfish blooms in coastal and estuarine waters around ... impact on marine food webs is likely to increase into ...
Cached Biology News:Using magnets to help prevent heart attacks 2Optimum tax on foreign fishing in Africa protects stocks and domestic fishing 2Jellyfish blooms shunt food energy from fish to bacteria 2Jellyfish blooms shunt food energy from fish to bacteria 3
(Date:9/20/2014)... (PRWEB) September 20, 2014 ... urological and interventional radiology products and services, has ... subsidiary with Clinicon Corporation for its ... HealthTronics’ portfolio of minimally invasive, mobile, surgical technology—offering ... , Under the agreement, HealthTronics will make available ...
(Date:9/19/2014)... to move data would consume much less power ... as chips, transistor counts rise. , Of the ... emitters, modulators, and detectors emitters are the ... optical chips is molybdenum disulfide (MoS2), which has ... atom-thick layer. Other experimental on-chip light emitters have ...
(Date:9/19/2014)... Sept. 19, 2014 Pfenex Inc. (NYSE MKT: ... of high-value and difficult to manufacture proteins including biosimilar ... the 21 st Annual NewsMakers in the Biotech ... Bertrand Liang , chief executive officer of Pfenex, ... and business strategy on Friday, September 26, 2014 at ...
(Date:9/19/2014)... , September 19, 2014 ... 53rd European Society for Paediatric Endocrinology (ESPE) Meeting  ... science and medical research in the field of growth  ... KGaA, Darmstadt, Germany , today announced ... (GGI) for 2014. The awards were announced during a ...
Breaking Biology Technology:HealthTronics Announces Distribution Agreement with Clinicon-Laser Surgical Device Expands Minimally Invasive, Mobile Technology Portfolio 2HealthTronics Announces Distribution Agreement with Clinicon-Laser Surgical Device Expands Minimally Invasive, Mobile Technology Portfolio 3Toward optical chips 2Toward optical chips 3Pfenex to Present at Upcoming Industry Conference on September 26 2EMD Serono Awards Grant for Growth Innovation (GGI) for the First Time 2EMD Serono Awards Grant for Growth Innovation (GGI) for the First Time 3
... DUBLIN and PHILADELPHIA , July 8, 2010 ... global specialty biopharmaceutical company,announces that its subsidiaries Shire Development Inc. ... U.S. District,Court for the District of Delaware against ... ( USA ), Inc. (collectively,"Zydus") for the infringement of U.S. ...
... , CHICAGO , July 8 Advanced Life ... in the discovery, development and commercialization of novel drugs in the ... results from preclinical toxicology and pharmacokinetic studies of an intravenous (IV) ... a hospital setting. , , ...
... ... ... Oxford Performance Materials (OPM) is very pleased to announce that the firm’s OXPEKK-IG long-term ... KFDA approved the Viking interspinous spinal device made by OPM’s customer Sintea Plustek., , ...
Cached Biology Technology:Lialda(R) - Shire Files Suit Against Cadila Healthcare Limited, Doing Business as Zydus Cadila and Zydus Pharmaceuticals (USA), Inc. 2Lialda(R) - Shire Files Suit Against Cadila Healthcare Limited, Doing Business as Zydus Cadila and Zydus Pharmaceuticals (USA), Inc. 3Advanced Life Sciences Announces Successful Preclinical Data Supporting Restanza Intravenous Formulation for Hospital Use 2Advanced Life Sciences Announces Successful Preclinical Data Supporting Restanza Intravenous Formulation for Hospital Use 3Advanced Life Sciences Announces Successful Preclinical Data Supporting Restanza Intravenous Formulation for Hospital Use 4OXPEKK-IG Device Viking Gains Regulatory Approval in Korea 2OXPEKK-IG Device Viking Gains Regulatory Approval in Korea 3
... NeuroLog System is a compact, versatile & modular ... of purposes including: Intracellular, Extracellular, Transducer & ... The NeuroLog Case & Power Supply unit (NL900D) ... Modules are not only powered by this unit, ...
anti-Yan 8B12H9...
...
... T4 DNA Ligase catalyzes the formation ... ATP between double-stranded DNAs with 3' hydroxyl ... DNA Ligase buffer optimizes ligation which can ... nucleic acids are not substrates for this ...
Biology Products: