Navigation Links
A new spin on drug delivery
Date:7/9/2010

Blacksburg, Va., July 12, 2010 Chang Lu and his chemical engineering research group at Virginia Tech have discovered how to "greatly enhance" the delivery of DNA payloads into cells. The description of their work will be featured on the cover of Lab on a Chip (issue 16), the premier journal for researchers in microfluidics. http://www.rsc.org/Publishing/Journals/LC/article.asp?doi=c004472e

The work also appears in the July 8 issue of Nature magazine (Vol. 466, p. 163) http://www.nature.com/nature/journal/v466/n7303/pdf/466163d.pdf

Lu's ultimate goal is to apply this technique to create genetically modified cells for cancer immunotherapy, stem cell therapy and tissue regeneration.

One of the most widely used physical methods to deliver genes into cells "is incredibly inefficient because only a small fraction of a cell's total membrane surface can be permeated," said Lu, an associate professor of chemical engineering at Virginia Tech. http://www.microfluidics.che.vt.edu/

The method Lu is referring to is called electroporation, a phenomenon known for decades that increases the permeability of a cell by applying an electric field to generate tiny pores in the membrane of cells.

Lu called the process "a new spin on DNA delivery." He explained the process saying, "Conventional electroporation methods deliver DNA only within a very small portion of the cell surface, determined by the physics governing the interaction between an electric field and a cell. Our method enables uniform DNA delivery over the entire cell surface, which is the first time we are aware that this has been demonstrated. The result is a greatly enhanced transfer of the genetic material."

Lu said his new approach harnesses "hydrodynamic effects that uniquely occur when fluids flow along curved paths. Flow under these conditions is known to generate vortices. Cells carried by such flow experience rotation and spinning that help expose the majority area of its surface to the electric field. " Having the gene delivery done by flows in curved paths is key in the gene delivery as opposed to the traditionally used, electroporation in static solution or in straight channels. "A spiral-shaped channel design yields a two-fold increase than a straight channel and an even larger factor compared to in static solution," he added.

By using fluorescence microscopy, they were able to "map" the area on the cell surface that was subjected to electroporation, and determine the extent of the DNA entry into the cell.

Lu explained the conventional delivery using a cuvette-type of device with static cell suspension produces DNA delivery confined to a narrow zone on the cell surface. However, when electroporation is applied to flowing cells in a spiral or curved channel , the images "appear dramatically different with the DNA delivery uniformly distributed over the entire cell surface."


'/>"/>

Contact: Lynn Nystrom
tansy@vt.edu
540-231-4371
Virginia Tech
Source:Eurekalert  

Related biology news :

1. Researchers develop drug delivery system using nanoparticles triggered by electromagnetic field
2. Geisinger Implements MedEx System to Enhance Medication Delivery, Tracking
3. Air pollution doesnt increase risk of preeclampsia, early delivery, study finds
4. Importance of insulin delivery devices for diabetes management
5. Multistage nanovector system provides sustained delivery of siRNA cancer therapeutic in mice
6. ViroPharma Announces Initiation of Phase 2 Evaluation of Subcutaneous Delivery of Cinryze(TM) (C1 Esterase Inhibitor [Human])
7. Phoenix Solar AG and MiaSole Sign Multi-Year Framework Agreement for the Delivery of Thin-Film Solar Modules
8. Pitt-led international study identifies human enzyme that breaks down potentially toxic nanomaterials, opens door to novel drug delivery
9. Cancer therapy using unique imaging, delivery system focus of NSF CAREER Award
10. UC-developed technology to assist research and genetic drug delivery
11. Lower income women report more insurance-based discrimination during pregnancy, delivery
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
A new spin on drug delivery
(Date:5/16/2017)... May 16, 2017  Veratad Technologies, LLC ( www.veratad.com ... age and identity verification solutions, announced today they will ... 2017, May 15 thru May 17, 2017, in ... International Trade Center. Identity impacts the ... in today,s quickly evolving digital world, defining identity is ...
(Date:4/19/2017)... The global military biometrics market ... by the presence of several large global players. The ... major players - 3M Cogent, NEC Corporation, M2SYS Technology, ... 61% of the global military biometric market in 2016. ... military biometrics market boast global presence, which has catapulted ...
(Date:4/13/2017)... According to a new market research report "Consumer IAM Market by ... Service, Authentication Type, Deployment Mode, Vertical, and Region - Global Forecast to ... USD 14.30 Billion in 2017 to USD 31.75 Billion by 2022, at ... ... MarketsandMarkets Logo ...
Breaking Biology News(10 mins):
(Date:10/11/2017)... ... October 11, 2017 , ... ComplianceOnline’s Medical Device Summit is ... and 8th June 2018 in San Francisco, CA. The Summit brings together current and ... distinguished CEOs, board directors and government officials from around the world to address key ...
(Date:10/11/2017)... ... ... Disappearing forests and increased emissions are the main causes of the evolving air ... living in larger cities are affected by air pollution related diseases. , That is ... globally - decided to take action. , “I knew I had to take action ...
(Date:10/10/2017)... ... October 10, 2017 , ... ... antibody-drug conjugate (ADC) therapeutics, today confirmed licensing rights that give it exclusive ... a technology developed in collaboration with Children’s Hospital Los Angeles (CHLA). ...
(Date:10/10/2017)... SomaGenics announced the receipt of a Phase ... (Single Cell), expected to be the first commercially available ... from single cells using NGS methods. The NIH,s recent ... development of approaches to analyze the heterogeneity of cell ... for measuring levels of mRNAs in individual cells have ...
Breaking Biology Technology: