Navigation Links
A new cost-effective genome assembly process
Date:5/5/2013

The U.S. Department of Energy Joint Genome Institute (DOE JGI) is among the world leaders in sequencing the genomes of microbes, focusing on their potential applications in the fields of bioenergy and environment. As a national user facility, the DOE JGI is also focused on developing tools that more cost-effectively enable the assembly and analysis of the sequence that it, as well as other genome centers, generates.

Despite tremendous advances in cost reduction and throughput of DNA sequencing, significant challenges remain in the process of efficiently reconstructing genomes. Existing technologies are good at cranking out short fragments (reads) of DNA letters that are computationally stitched back together (assembled) into longer pieces, so that the order of those letters can be determined and the function of the target sequence discerned. However, genome assembly, the equivalent of trying to put together a multi-million piece jigsaw puzzle without knowing what the picture on the cover of the box is, remains challenging due to the very large number of very small pieces, which must be assembled using current approaches.

As reported May 5 online in the journal Nature Methods, a collaboration between the DOE JGI, Pacific Biosciences (PacBio) and the University of Washington has resulted in an improved workflow for genome assembly that the team describes as a fully automated process from DNA sample preparation to the determination of the finished genome.

The technique, known as HGAP (Hierarchical Genome Assembly Process), uses PacBios single molecule, real-time DNA sequencing platform, which generates reads that can be up to tens of thousands of nucleotides long, even longer than those provided by the workhorse technology of the Human Genome Project era, the Sanger sequencing technology, which produced reads of about 700 nucleotides. The Sanger process involved creating multiple DNA libraries, conducting multiple runs, and combining th
'/>"/>

Contact: David Gilbert
degilbert@lbl.gov
925-296-5643
DOE/Joint Genome Institute
Source:Eurekalert

Page: 1 2 3

Related biology news :

1. Cost-effective production of infrared lenses
2. Turtle genome analysis sheds light on the development and evolution of turtle-specific body plan
3. CNIO researchers capture the replication of the human genome for the first time
4. Saint Louis University, University of Toronto biologists help decode turtle genome
5. Genome study suggests new strategies for understanding and treating pulmonary fibrosis
6. Coelacanth genome surfaces
7. Genome sequencing of the living coelacanth sheds light on the evolution of land vertebrate
8. Einstein joins the New York Genome Center as 12th institutional founding member
9. Scientists decode genome of painted turtle, revealing clues to extraordinary adaptations
10. Getting under the shell of the turtle genome
11. Peach genome offers insights into breeding strategies for biofuels crops
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:12/11/2014)... SALEM, N.C. , Dec. 10, 2014  That blood ... for quite a while. Hypertension – the medical term for ... in the early 1800s, and the inflatable cuff that,s used ... That doesn,t, however, mean there,s nothing new about hypertension, its ... some long-held beliefs about the condition and the best ways ...
(Date:12/10/2014)... -- CIE San Diego has launched an innovative trust ... that enhances care coordination among social service and care ... earned a second $1 million grant from the Alliance ... in community and; will be recognized as a "Live ... 4-6p. CIE San Diego today announced the ...
(Date:12/4/2014)... 3, 2014  Crossmatch™, a leading provider of ... DigitalPersona ® Pro Enterprise software and U.are.U ... throughout First Bank branch locations in ... and Virginia. First Bank, a progressive community ... North Carolina , selected the Crossmatch biometrics-based ...
Breaking Biology News(10 mins):Research points to need for new approaches to treatment of high blood pressure 2Research points to need for new approaches to treatment of high blood pressure 3Research points to need for new approaches to treatment of high blood pressure 4Community Information Exchange San Diego Launches Pilot, Secures $1 Million Expansion Grant, and Becomes a "Live Well San Diego" Partner 2Community Information Exchange San Diego Launches Pilot, Secures $1 Million Expansion Grant, and Becomes a "Live Well San Diego" Partner 3Community Information Exchange San Diego Launches Pilot, Secures $1 Million Expansion Grant, and Becomes a "Live Well San Diego" Partner 4First Bank Partners with Crossmatch to Increase Log In Security 2First Bank Partners with Crossmatch to Increase Log In Security 3
... could help deliver drugs and shape the growth of ... supermodel lips. But by mating collagen with a molecular ... some important medical advances. The researchers have found a ... for better infection-fighting bandages and a treatment to block ...
... bit of biology called site-specific recombination, DNA can travel inside ... of grafting surgery that allows it to insert its chromosome ... important because mobile DNA can carry genes that cause drug ... result in certain leukemias or other cancers. , But what ...
... blindness in older adults, yet researchers are still in ... this incurable disease. , But new insight from University ... between rhesus monkeys with macular degeneration and humans could ... when severe vision loss could still be stopped. , ...
Cached Biology News:Unusual antibiotics show promise against deadly 'superbugs' 2Unusual antibiotics show promise against deadly 'superbugs' 3Brown-Harvard team solves mobile DNA's surgical sleight-of-hand 2Genetic links could unlock clues to leading cause of blindness 2Genetic links could unlock clues to leading cause of blindness 3
(Date:12/24/2014)... BEIJING , Dec. 23, 2014 ... or the "Company"), a leading fully integrated plasma-based ... today announced that its majority-owned subsidiary, Shandong Taibang ... Practices ("GMP") certification from the China Food and ... factor production facility. As previously disclosed in the ...
(Date:12/24/2014)... PharmaBoardroom,s new report, , Healthcare and ... free download , digs deep into this vibrant market, ... sector today. One area where Algeria ... a homegrown pharmaceutical manufacturing base, even if the government,s ambitious ... off. A cursory comparison with neighboring Morocco ...
(Date:12/24/2014)... , Dec. 23, 2014  Rock Creek Pharmaceuticals, ... it has filed a Clinical Trial Application (CTA) with ... products Regulatory Agency (MHRA) seeking regulatory approval to initiate ... molecule, Anatabine Citrate. Contingent on the Company ... a Phase I trial to assess the safety, tolerability ...
(Date:12/22/2014)... 2014 The Center for Professional ... technical training across the life sciences industry, has partnered ... (SCDM) to provide the organization's members with discounted classroom ... to the more than 350 sessions across 80 course ... off when registering for a public course ...
Breaking Biology Technology:China Biologic Receives GMP Certification for New Coagulation Factor Facility 2China Biologic Receives GMP Certification for New Coagulation Factor Facility 3PharmaBoardroom Releases New Algeria Pharmaceuticals Report 2PharmaBoardroom Releases New Algeria Pharmaceuticals Report 3Rock Creek Pharmaceuticals Files Clinical Trial Application in the UK for Clinical Trials of Anatabine Citrate Formulations 2Rock Creek Pharmaceuticals Files Clinical Trial Application in the UK for Clinical Trials of Anatabine Citrate Formulations 3CfPIE Announces Partnership with the Society for Clinical Data Management 2CfPIE Announces Partnership with the Society for Clinical Data Management 3
... from roadside bombs to freeway crashes. Traumatic injury, the leading ... overwhelms the body,s natural blood-clotting process. In an effort ... Lavik, a new Case Western Reserve University biomedical engineering professor, ... platelets that show promise in halting internal and external bleeding. ...
... , Nursing Care at Hahnemann among the Best ... University Hospital proudly announces it has been granted Magnet(R) recognition ... Magnet(R) is one of the highest levels of recognition a ... the five percent of hospitals in the country that have ...
... , , , , ... of the world,s leading research and advisory firms for ... from surveyed dermatologists, Centocor Ortho Biotech/Janssen-Cilag,s Stelara and Abbott,s ... the biologics share in 2012 in the drug market ...
Cached Biology Technology:Researchers take the inside route to halt bleeding 2Hahnemann University Hospital Granted Magnet(R) Status 2Hahnemann University Hospital Granted Magnet(R) Status 3In 2012, Stelara and Briakinumab Will Capture 22 Percent of the Biologics Share in the Moderate to Severe Psoriasis Drug Market 2In 2012, Stelara and Briakinumab Will Capture 22 Percent of the Biologics Share in the Moderate to Severe Psoriasis Drug Market 3
... Labeling Kit with CyScribe GFX Purification ... and purification of Cy3- and Cy5-labeled ... complete cDNA labeling and purification.Flexible and ... performing labeling reactions with either Cy3- ...
... CyScribe Post-Labeling Kit with CyScribe GFX ... generation and purification of Cy3- and ... for complete cDNA labeling and purification.Flexible ... for performing labeling reactions with either ...
Collected from sexually mature, mixed sex, mixed breed dogs. Serum from specific breeds available by request....
UV-NIR light source for USB2000, HR4000, HR2000+...
Biology Products: