Navigation Links
A mold on which to create new parts of the puzzle that is the nervous system
Date:8/16/2011

Nervous system diseases (such as Parkinson's or post-traumatic medullar injury) are especially difficult to treat, as it is not easy to replace the parts of the neural puzzle which are damaged. The key is in developing functional neurons from in vitro-treated, cells but for this it is essential that the support on which these cells are based simulate the characteristics of the nervous system. This is what biochemist Patricia Garca has done, developing and validating a polymer support capable of inducing neuronal differentiation in vitro. Her PhD thesis, defended at the University of the Basque Country (UPV/EHU) and undertaken at the Tecnalia Health Unit, is entitled Development of a new polymer support with components of extracellular neural matrix for application in the in vitro differentiation of different primary cells.

Ms Garca has developed a polymer material which contains topographic and biochemical characteristics suitable for converting cells into functional neurones. With this purpose in mind, photolithographic techniques have been combined with the technology of extracellular neural matrixes. The photolithographic technique involves a series of treatments based on exposure to light and chemical substances, and has acted to provide the support with the desired topographical form. As regards the extracellular neural matrix, cells adhere to this natural substrate in order to, amongst other things, be guided and acquire neuronal functionality, enabling the formation of new transplantable tissue for the nervous system. Ms Garca simulated the properties of this matrix in the support developed.

Biocompatible and inductor

At the validation phase, this polymer material proved to be biocompatible with the nervous system. It also acts to induce the formation of neurones sufficiently developed to be able to be used therapeutically, providing the induction is carried out from neural origin cells (the trials with non-neural origin cells gave rise to immature neurones, in the best of cases). Ms Garca undertook validations with the PC12 rat established cell line (used in numerous trials for neuronal differentiation) and with cell cultures of different origins (primary cultures) extracted from rats.

Concretely, in the case of the validations with PC12, the biocompatibility and capacity of neuronal and dopaminergic differentiation (dopamine complies with neurotransmitter functions) of the support developed have been clearly demonstrated.

Only with those of neural origin

As regards primary cultures, Ms Garca carried out trials with cells from the hypocampus in the embryonic rat. These are cells not only of neural origin but with the neuronal line already determined in states of early development such as the embryonic stage. These cells differentiate more efficiently in the developed matrix than in the classic conditions of culture, obtaining mature neurones after only eight days.

The second primary culture validated in the matrix was the one consisting of neural precursor cells (cells at the stage prior to converting into neurones) of the subventricular zone (also the brain) of the neonatal rat. The results show the capacity of dopaminergic neuronal differentiation in less than 30 days of culture. Moreover, an extensive network of glial cells between the matrix and the neurones were observed, and whose function is to give trophic support to and signal the neurones in development.

The last validation trial in primary culture was undertaken with cells of non-neural origin. These are precursor cells derived from the skin of an adult rat, given that they have been already described in the literature as an important source of neural precursor cells. Nevertheless, in this case, the cells stay undifferentiated on the matrix developed, and only in a low percentage have immature neurones been obtained.


'/>"/>

Contact: Amaia Portugal
a.portugal@elhuyar.com
34-943-363-040
Elhuyar Fundazioa
Source:Eurekalert

Related biology news :

1. USC scientists uncover mechanism by which chronic stress causes brain disease
2. How ants tame the wilderness: Rainforest species use chemicals to identify which plants to prune
3. Biodiversity in danger: Which areas should be protected?
4. Insects hold atomic clues about the type of habitats in which they live
5. Microchip patented which separates and extracts tumor cells in the blood
6. La Jolla Institute scientist leads team which discovers important new player in diabetes onset
7. Research in which the University of Navarra participated provides new clues to causes of leukemia
8. Reprogram stem cells with heart tissue, which might improve treatments for heart disease
9. New way discovered to predict which breast cancer patients should be treated with anthracyclines
10. Which came first: Religion or the brain?
11. Yale researchers may have uncovered the mechanism by which progesterone prevents preterm birth
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:2/2/2016)... NEW YORK , Feb. 2, 2016 /PRNewswire/ ... Potentials of that Rising Market Are you ... new analysis forecasts revenues for checkpoint inhibitors. Visiongain,s ... world market, submarket, product and national level. ... Instead discover what progress, opportunities and revenues those ...
(Date:2/2/2016)... , Feb. 2, 2016   Parabon NanoLabs ... the U.S. Army Research Office and the Defense ... and sensitivity of the company,s Snapshot Kinship ... Mission and, more generally, defense-related DNA forensics.  Although ... capabilities (predicting appearance and ancestry from DNA evidence), ...
(Date:2/1/2016)... , February 1, 2016 ... advancements to drive global touchfree intuitive gesture control market ... --> Rising sales of consumer electronics coupled with ... control market size through 2020 ... electronics coupled with new technological advancements to drive global ...
Breaking Biology News(10 mins):
(Date:2/9/2016)... ... February 08, 2016 , ... Date ... S. Blumberg Institute at the Pennsylvania Biotechnology Center of Bucks County, 3805 Old ... Commonwealth Medical College (TCMC) will hold an open house for participants to learn ...
(Date:2/9/2016)... ... 2016 , ... The American Academy of Thermology (AAT) has announced ... AAT Member Certification Qualification Course for Technicians via a two part webinar on July ... a detailed review of hardware, software, and camera setup/operations, aligns with the in-person member ...
(Date:2/9/2016)... NEW YORK , Feb. 9, 2016 ... market analyzes the current and future prospects of the ... of this report include companies engaged in the manufacture ... comprises an executive summary with a market snapshot providing ... the scope of this report. This section also provides ...
(Date:2/9/2016)... , Feb. 9, 2016 ... Insights, 2016", report provides in depth insights ... activities around the Protein-Tyrosine Phosphatase 1B (PTP1B) ... profiles in various stages of development including ... Phase III and Preregistration. Report covers the ...
Breaking Biology Technology: