Navigation Links
A global breakthrough in the study of a protein linked to the spread of viruses

This release is available in French.

Montreal, January 5, 2010 Professor Denis Archambault of the Department of Biological Sciences of Universit du Qubec Montral (UQAM), and doctoral student Andrea Corredor Gomez have made a major discovery in the field of molecular biology. They have unlocked some of the secrets of a viral protein, known as Rev, which is very different from other proteins of the same type studied to date. The results of their research were recently published in the prestigious Journal of Virology.

The Rev protein plays an essential role in the propagation mechanism of certain types of viruses within an organism. The work of researchers Archambault and Gomez Corredor focused on this protein, and more particularly on a structure called the "nuclear localization signal" (NLS). They used as a model the Rev protein of the bovine immunodeficiency virus (BIV), a retrovirus related to the AIDS virus in humans.

Retroviruses, like all other viruses, are characterized by an inability to multiply on their own. In order to reproduce, they require a living host cell. A cell has a cytoplasm and a nucleus at the centre. The nucleus contains nucleoli, or sub-compartments. It was already known that the Rev protein, produced in the cytoplasm, moves into the nucleus and nucleoli of a cell infected with certain retroviruses. By binding to the viral RNAs found in the nucleus, it contributes to the transition of an infection from the early to the late stage. To fulfil this primary function, the Rev protein must first be able to enter the nucleus. To do so, it needs a "key", its "NLS" composed of amino acids.

A different nuclear localization signal (NLS)

Over the years, several researchers have looked at the NLS in different Rev proteins. Until now, the study of these proteins demonstrated the presence of a monopartite NLS, i.e. an NLS comprising one continuous sequence of amino acids. Much to their surprise, Denis Archambault and Andrea Gomez Corredor discovered that the BIV Rev protein has a bipartite NLS composed of two amino acid motifs separated by a sequence of additional amino acids a world first for this type of protein in all retroviruses studied to date, including the AIDS virus.

In addition, although other types of protein contain a bipartite NLS, this newly discovered NLS does not match any other bipartite NLS identified until now, whatever the type of protein studied. Normally a bipartite NLS is composed of two amino acid motifs separated by a spacer sequence, which is either short (about 10 amino acids) or long (about 30 amino acids). In the BIV Rev protein, the NLS is atypical because of the length of the spacer sequence (neither long nor short) and the amino acid composition of that sequence.

Finally, the authors also identified a new type of nucleolar localization signal (NoLS) which allows the Rev protein to penetrate inside the nucleoli. Although the role of this localization is unknown, it is the first time this type of signal has been reported in proteins of cellular or viral origin.

A first step toward further discoveries

According to Denis Archambault, "What we have here is a Rev protein whose characteristics are very different from the other proteins of the same type that have been studied to date. Although our findings relate to basic research, our study demonstrates that it is possible to learn a lot about viruses, and in particular a virus of animal origin. We now have a specific model that will allow us to study further the relationship between the localization of a protein and its effect on the host cell, and possibly the entire organism."


Contact: Claire Bouchard
Universit du Qubec Montral

Related biology news :

1. Global Warming and the Habitability of Planet Earth, Sept. 26
2. Green skies: Engineers work may reduce jet travels role in global warming
3. Scientists in first global study of poison gas in the atmosphere
4. North Americas northernmost lake affected by global warming
5. IEEE-USA innovation forum will help prepare US tech leaders to prosper in a global marketplace
6. Majority of Americans want local action on global warming, says poll
7. International team of scientists warns of climate changes impact on global river flow
8. Changing the global dietary environment
9. Global deal fuels QUTs world-changing research
10. NIH hosts event to launch Council of Science Editors global theme issue
11. Agricultural soil erosion is not adding to global warming
Post Your Comments:
(Date:11/17/2015)... PARIS , November 17, 2015 ... 17 au 19 novembre  2015.  --> Paris ... 2015.  --> DERMALOG, le leader de l,innovation ... à la fois passeports et empreintes sur la même ... pour les passeports et l,autre pour les empreintes digitales. ...
(Date:11/12/2015)... 12, 2015  A golden retriever that stayed healthy ... (DMD) has provided a new lead for treating this ... Broad Institute of MIT and Harvard and the University ... Cell, pinpoints a protective gene ... disease,s effects. The Boston Children,s lab of Lou ...
(Date:11/10/2015)... 10, 2015  In this report, the ... of product, type, application, disease indication, and ... report are consumables, services, software. The type ... biomarkers, efficacy biomarkers, and validation biomarkers. The ... diagnostics development, drug discovery and development, personalized ...
Breaking Biology News(10 mins):
(Date:11/24/2015)... ... November 24, 2015 , ... ... year and one of the premier annual events for pharmaceutical manufacturing: 2015 Annual ... November 2015, where ISPE hosted the largest number of attendees in more than ...
(Date:11/24/2015)... , Nov. 24, 2015  Clintrax Global, Inc., a worldwide ... Carolina , today announced that the company has set a ... a 391% quarter on quarter growth posted for Q3 of 2014 ... and Mexico , with the establishment of ... December 2015. --> United Kingdom and ...
(Date:11/24/2015)... , ... November 24, 2015 , ... This fall, global ... competitive events in five states to develop and pitch their BIG ideas to improve ... each state are competing for votes to win the title of SAP's Teen Innovator, ...
(Date:11/24/2015)... November 24, 2015 SHPG ) announced today ... the Piper Jaffray 27 th Annual Healthcare Conference in ... 2015, at 8:30 a.m. EST (1:30 p.m. GMT). --> ... Officer, will participate in the Piper Jaffray 27 th Annual ... on Tuesday, December 1, 2015, at 8:30 a.m. EST (1:30 p.m. ...
Breaking Biology Technology: