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A 'clear' choice for clearing 3-D cell cultures
Date:9/3/2014

eeDB made them smaller and didn't improve clarity as much.

Maintaining the tissue culture size is important because Boutin wants to know the physical dimensions of growth in the culture, such as the axons that one neuron might extend to another.

While ClearT2 worked within 1.5 hours, Scale and SeeDB took three days, Boutin said.

She ran several further tests with ClearT2, including with other types of healthy and cancerous neural cells, and ClearT2 continued to perform well, even allowing her to image extracellular matrix.

In the paper Boutin and Hoffman-Kim acknowledge that the results with different methods may vary with different samples, but they expect that for many "scaffold-free" engineered 3-D neural tissues tissues that grow without added matrix supports ClearT2 should work well.

Knowing that could clear the way for many research projects with 3-D tissues.


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Contact: David Orenstein
david_orenstein@brown.edu
401-863-1862
Brown University
Source:Eurekalert

Page: 1 2

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