Navigation Links
A chaperone system guides tail-anchored membrane proteins to their destined membrane
Date:7/5/2011

This release is available in German.

FRANKFURT. A newly synthesized protein is as fragile as a newborn baby. It could never fold into its correct three dimensional structure if it was not protected by chaperones within the densely populated cytosol. In case of membrane proteins chaperones do not only pre-vent their aggregation, but also escort them to their destination and aid in membrane insertion. The underlying molecular mechanism of how a certain family of membrane proteins is targeted and inserted into membranes has now been resolved by an international research team with participation of the Goethe University Frankfurt. These proteins are anchored within the membrane via a single helix and thus are called "tail-anchored" (TA) proteins.

The key for proper protein sorting are signal sequences which are decoded by chaperones. As soon as they - together with their "foster child" - arrive at their destination the interaction with a specific receptor within the target membrane initiates membrane insertion. Protein components responsible for the insertion of TA proteins have recently been identified. The molecular mechanisms of how these sorting systems work, however, were not known so far.

In this interdisciplinary study, that will be released in the current "online" issue of "Science", the research groups of Prof.Volker Dtsch (Goethe University Frankfurt), Prof. Irmgard Sinning (Heidelberg University Biochemistry Center) and Prof. Vlad Denic (Harvard University (USA)) were able to solve the question by a combination of diverse methods such as X-Ray Crystallography and NMR-Spectroscopy as well as biochemical and cell-biological approaches.

In detailed biophysical studies Volker Dtschs research group showed that the central cha-peron of the responsible protein complex, called Get3, regulates both binding to TA proteins within the cytosol and their release at the membrane. The two receptor proteins Get1 and Get2 aid in TA protein insertion. They use overlapping interfaces for the interaction with the Get3 ATPase. On the basis of different crystal structures the researchers suggest a model for the mechanism of how TA proteins are inserted into the membrane. Upon interaction with its membrane receptor the Get3 dimer gradually opens up to allow for the controlled TA protein insertion. "Those results are particularly important because they enabled us to establish the first model of the receptor-assisted membrane insertion of TA proteins that will now be the basis for further studies" comments Dtsch.

Publication Stefer, S. et al: Structural basis for tail-anchored membrane protein biogenesis by the Get3-receptor complex, Science (2011), DOI: 10.1126/science.1207125.


'/>"/>
Contact: Prof. Volker Dtsch
vdoetsch@em.uni-frankfurt.de
49-697-982-9631
Goethe University Frankfurt
Source:Eurekalert  

Related biology news :

1. Common chaperone protein found to work in surprising way, say Scripps Research scientists
2. Blame the chaperone
3. Dynamics of chaperone protein critical in rescuing brains of Alzheimers mice from neuron damage
4. Breakthrough: With a chaperone, copper breaks through
5. Molecular chaperone keeps bacterial proteins from slow-dancing to destruction
6. Researchers decipher protein structure of key molecule in DNA transcription system
7. Global plant database set to promote biodiversity research and Earth-system sciences
8. Lockheed Martins HULCâ„¢ Robotic Exoskeleton Enters Biomechanical Testing at U.S. Army Natick Soldier Systems Center
9. U of M researchers contribute to global plant database, expanding ecosystems research
10. Twin-head cucumber system reduces start-up costs
11. China needs improved administrative system for nuclear power safety
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
A chaperone system guides tail-anchored membrane proteins to their destined membrane
(Date:6/27/2016)... Research and Markets has announced the addition of the ... The report forecasts the biometrics ... a CAGR of 12.28% during the period 2016-2020. ... with inputs from industry experts. The report covers the market landscape ... includes a discussion of the key vendors operating in this market. ...
(Date:6/22/2016)... , June 22, 2016  The American College of Medical ... Show Executive Magazine as one of the fastest-growing trade ... 25-27 at the Bellagio in Las Vegas ... highest percentage of growth in each of the following categories: ... companies and number of attendees. The 2015 ACMG Annual Meeting ...
(Date:6/22/2016)... LOS ANGELES , June 22, 2016 ... of identity management and verification solutions, has ... cutting edge software solutions for Visitor Management, ... ® provides products that add functional ... The partnership provides corporations and venues with ...
Breaking Biology News(10 mins):
(Date:6/24/2016)... June 24, 2016 Epic Sciences unveiled ... cancers susceptible to PARP inhibitors by targeting homologous ... (CTCs). The new test has already been incorporated ... multiple cancer types. Over 230 clinical ... response pathways, including PARP, ATM, ATR, DNA-PK and ...
(Date:6/23/2016)... , June 23, 2016   Boston Biomedical ... novel compounds designed to target cancer stemness pathways, ... been granted Orphan Drug Designation from the U.S. ... of gastric cancer, including gastroesophageal junction (GEJ) cancer. ... designed to inhibit cancer stemness pathways by targeting ...
(Date:6/23/2016)... , June 23, 2016  The Prostate Cancer Foundation (PCF) is ... treatments and faster cures for prostate cancer. Members of the Class of 2016 ... countries. Read More About the Class of 2016 PCF ... ... ...
(Date:6/23/2016)... ... 2016 , ... In a new case report published today in STEM CELLS ... developed lymphedema after being treated for breast cancer benefitted from an injection of stem ... with this debilitating, frequent side effect of cancer treatment. , Lymphedema refers ...
Breaking Biology Technology: