Navigation Links
A chaperone system guides tail-anchored membrane proteins to their destined membrane
Date:7/5/2011

This release is available in German.

FRANKFURT. A newly synthesized protein is as fragile as a newborn baby. It could never fold into its correct three dimensional structure if it was not protected by chaperones within the densely populated cytosol. In case of membrane proteins chaperones do not only pre-vent their aggregation, but also escort them to their destination and aid in membrane insertion. The underlying molecular mechanism of how a certain family of membrane proteins is targeted and inserted into membranes has now been resolved by an international research team with participation of the Goethe University Frankfurt. These proteins are anchored within the membrane via a single helix and thus are called "tail-anchored" (TA) proteins.

The key for proper protein sorting are signal sequences which are decoded by chaperones. As soon as they - together with their "foster child" - arrive at their destination the interaction with a specific receptor within the target membrane initiates membrane insertion. Protein components responsible for the insertion of TA proteins have recently been identified. The molecular mechanisms of how these sorting systems work, however, were not known so far.

In this interdisciplinary study, that will be released in the current "online" issue of "Science", the research groups of Prof.Volker Dtsch (Goethe University Frankfurt), Prof. Irmgard Sinning (Heidelberg University Biochemistry Center) and Prof. Vlad Denic (Harvard University (USA)) were able to solve the question by a combination of diverse methods such as X-Ray Crystallography and NMR-Spectroscopy as well as biochemical and cell-biological approaches.

In detailed biophysical studies Volker Dtschs research group showed that the central cha-peron of the responsible protein complex, called Get3, regulates both binding to TA proteins within the cytosol and their release at the membrane. The two receptor proteins Get1 and Get2 aid in TA protein insertion. They use overlapping interfaces for the interaction with the Get3 ATPase. On the basis of different crystal structures the researchers suggest a model for the mechanism of how TA proteins are inserted into the membrane. Upon interaction with its membrane receptor the Get3 dimer gradually opens up to allow for the controlled TA protein insertion. "Those results are particularly important because they enabled us to establish the first model of the receptor-assisted membrane insertion of TA proteins that will now be the basis for further studies" comments Dtsch.

Publication Stefer, S. et al: Structural basis for tail-anchored membrane protein biogenesis by the Get3-receptor complex, Science (2011), DOI: 10.1126/science.1207125.


'/>"/>
Contact: Prof. Volker Dtsch
vdoetsch@em.uni-frankfurt.de
49-697-982-9631
Goethe University Frankfurt
Source:Eurekalert  

Related biology news :

1. Common chaperone protein found to work in surprising way, say Scripps Research scientists
2. Blame the chaperone
3. Dynamics of chaperone protein critical in rescuing brains of Alzheimers mice from neuron damage
4. Breakthrough: With a chaperone, copper breaks through
5. Molecular chaperone keeps bacterial proteins from slow-dancing to destruction
6. Researchers decipher protein structure of key molecule in DNA transcription system
7. Global plant database set to promote biodiversity research and Earth-system sciences
8. Lockheed Martins HULC™ Robotic Exoskeleton Enters Biomechanical Testing at U.S. Army Natick Soldier Systems Center
9. U of M researchers contribute to global plant database, expanding ecosystems research
10. Twin-head cucumber system reduces start-up costs
11. China needs improved administrative system for nuclear power safety
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
A chaperone system guides tail-anchored membrane proteins to their destined membrane
(Date:4/13/2017)... MONICA, Calif. , April 13, 2017 ... New York will feature emerging and evolving ... Summits. Both Innovation Summits will run alongside the expo ... of speaker sessions, panels and demonstrations focused on trending ... coast,s largest advanced design and manufacturing event will take ...
(Date:4/11/2017)... 2017 NXT-ID, Inc. (NASDAQ:   NXTD ... the appointment of independent Directors Mr. Robin D. Richards ... of Directors, furthering the company,s corporate governance and expertise. ... Gino Pereira , Chief ... to their guidance and benefiting from their considerable expertise as ...
(Date:4/4/2017)... YORK , April 4, 2017   EyeLock ... today announced that the United States Patent and Trademark ... patent broadly covers the linking of an iris image ... same transaction) and represents the company,s 45 th ... latest patent is very timely given the multi-modal biometric ...
Breaking Biology News(10 mins):
(Date:4/28/2017)... ... April 28, 2017 , ... ... has validated a 0.2 ng/mL lower limit (LLOQ) assay for nicotine and two ... LLOQ assay, the ultra-low trace nicotine assay meets additional needs of tobacco industry ...
(Date:4/27/2017)... ... 2017 , ... Oncolinx, a spin-out of the Center for ... taken their cancer research out of this world. On April 22, 2017, an ... the International Space Station’s (ISS) U.S. National Laboratory, managed by the Center for ...
(Date:4/27/2017)... ... , ... Mitotech S.A, a Luxembourg based clinical stage biotechnology company, announced positive ... a rare devastating genetic disease that leads to a sudden and rapid loss of ... patients carrying 11778, 14484 and 3460 mutations and having experienced the onset of symptoms ...
(Date:4/27/2017)... ... April 27, 2017 , ... Arrowhead ... ( http://www.paintherapeuticsummit.com ) is coming to San Diego, CA on September 27-28, 2017. ... learn about the latest advances in the treatment of various types of pain. ...
Breaking Biology Technology: