Navigation Links
A boost for cellular profiling
Date:9/22/2013

New York, NY and Stockholm, Sweden -- A team of researchers affiliated with Ludwig Cancer Research and the Karolinska Institutet in Sweden report in the current issue of Nature Methods a dramatically improved technique for analyzing the genes expressed within a single cell -- a capability of relevance to everything from basic research to future cancer diagnostics.

"There are cells in tumors and in healthy tissues that are not present in sufficient numbers to permit analysis using anything but single-cell methods," explains senior author, Rickard Sandberg, PhD. "This method allows us to identify rare and important subpopulations of cells in all sorts of tissues. We can also use it to tease apart, more rigorously than ever before, how the expression of unique suites of genes transform cells from one state to another as, say, an embryo develops into an organism, or a tumor becomes metastatic."

Traditional approaches, which depend on the collective analysis of gene expression in millions of cells at once, tend to obscure biologically significant differences in the genes expressed by specialized cells within a particular kind of tissue. Single-cell analysis of gene expression overcomes this limitation. The leading method for such analysis -- Smart-seq -- was developed in 2012 by the biotechnology firm Illumina, together with Sandberg's laboratory.

To develop the new technique, named Smart-seq2, Sandberg's team conducted more than 450 experiments to improve upon their initial method. The new procedure consistently captures three to four times as many RNA molecules, which often translates into 2,000 more genes per cell than current methods allow. It also captures far more full-length gene sequences, a steep challenge in such studies, which often capture only partial sequences of expressed genes
'/>"/>

Contact: Rachel Steinhardt
rsteinhardt@licr.org
212-450-1582
Ludwig Institute for Cancer Research
Source:Eurekalert

Page: 1 2

Related biology news :

1. New Brain Supplement Focus Boost Launches, Boosting Alertness, Memory and Focus
2. Playing video games can boost brain power
3. Vitamins and minerals can boost energy and enhance mood
4. Immune-boosting colorectal cancer drug shows promise
5. Unexpected discovery of the ways cells move could boost understanding of complex diseases
6. Herbal extract boosts fruit fly lifespan by nearly 25 percent, UCI study finds
7. Improving crop per drop could boost global food security and water sustainability
8. Fast-sinking jellyfish could boost the oceans uptake of carbon dioxide
9. Quit smoking? Vitamin E may give extra boost to heart health
10. Green spaces may boost well-being for city slickers
11. Vaccine adjuvant uses host DNA to boost pathogen recognition
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:1/22/2015)... 2015 /PRNewswire/ - Today, FindBiometrics, the leading source of biometrics industry ... new website design. "When we launched FindBiometrics 12 ... Peter O,Neill , founder and CEO of FindBiometrics. "Now it,s ... from the key players on a very broad scale.  We ...
(Date:1/22/2015)...  BellBrook Labs, a leader in high throughput screening ... a TR-FRET (time resolved Forster resonance energy transfer) ... high throughput screening assay for glycosyltransferases and other ... sensitive detection of hundreds of human and microbial ...
(Date:1/22/2015)... Washington , January 20, 2015 Investorideas.com, ... tech stocks, releases video from the CES 2015 (Consumer Electronics ... Apollo Robbins . Apollo shows how easily ... smart wallet, a product of NXT-ID, Inc. (NASDAQ: ...
Breaking Biology News(10 mins):Identity Industry Leading Publication Debuts New Look As Biometrics Hit Mainstream 2BellBrook Labs Launches a High Throughput Screening Assay for Glycosyltransferases with TR-FRET Readout 2Apollo Robbins Talks about the Illusion of Personal Safety and How Easily Your Wallet is Stolen; If a Wocket Wallet is Stolen the Thief Can't do Anything With it! 2Apollo Robbins Talks about the Illusion of Personal Safety and How Easily Your Wallet is Stolen; If a Wocket Wallet is Stolen the Thief Can't do Anything With it! 3
... and Research Director Yrj Helariutta has discovered the genetic process ... of plants. Wood is the vascular tissue that transports water ... formation of the woody element in the stem. The work, ... Biology journal, presents a potential method for engineering plants ...
... Recently the number of genes known to be associated ... the MS4A gene cluster on chromosome 11. New research published ... has expanded on this using a genome-wide association study (GWAS) ... which is associated with Alzheimer,s disease. Alzheimer,s disease ...
... from the Walter and Eliza Hall Institute have overturned ... is controlled, solving the structure of a protein that ... be a potential drug target for future malaria and ... factor 1 (ADF1), a key protein involved in controlling ...
Cached Biology News:Researchers from the Viikki Biocenter discover how plants control the formation of wood cells 2Arrowing in on Alzheimer's disease 2New malaria protein structure upends theory of how cells grow and move 2New malaria protein structure upends theory of how cells grow and move 3
(Date:1/22/2015)... 22, 2015 Shimadzu Scientific Instruments ... multipurpose UV-visible spectrophotometer. Offering wavelength scanning from 190 ... ideal for applications in a variety of industries, ... The user-friendly UV-1280 enables intuitive operation, while the ...
(Date:1/22/2015)... NH (PRWEB) January 22, 2015 GEA ... a table top laboratory homogenizer, the PandaPLUS 2000, which ... nanodispersions, nanoemulsions , and cell disruption . This ... dairy products, fruit juices, liquid food, food additives and ...
(Date:1/22/2015)... 21, 2015 Cambridge Semantics, the leading provider ... announced that 2014 was a record-breaking year across the board ... the Anzo Smart Data Platform and our Smart Data solutions, ... from diverse data which led to record growth for the ...
(Date:1/22/2015)... Protocol Networks brings independent technology consulting ... brand-neutral, independent consultants, Protocol Networks has recently announced its ... the years, his company has attracted several clients in ... as well as others. With the success of these ...
Breaking Biology Technology:Shimadzu’s New Monitored Single-Beam UV-Vis Spectrophotometer Offers Comprehensive Measurement Options in a Compact Body 2GEA Niro Soavi Announces the PandaPLUS 2000 Homogenizer for Processing New Applications for Nanoemulsions and Cell Disruption 2Cambridge Semantics Announces Record Results for 2014 2Protocol Networks Brings Independent Technology Consulting to the Constitution State 2
... continue to increase the need for testing contaminants thereby generating ... look bright with the 2012 figure expected to touch $2.4 ... diagnostics. Microbiology testing market represents the largest and fastest growth ... ...
... elective and Orthopaedic admissions patients, in only ... DORSET, England, Nov. 4 Dorset County Hospital (DCH) ... of that plan, has,introduced enhanced MRSA surveillance. As part ... admissions for MRSA. Screening,enables hospitals to identify patients who ...
... MannKind Corporation,(Nasdaq: MNKD ), focused on discovering, ... that it will present at,the Rodman & Renshaw Annual ... AM (EST) at the New York Palace Hotel in ... to the live web cast of the,presentation in the ...
Cached Biology Technology:Global Agricultural and Environmental Diagnostics Market to Reach $2.4 Billion by 2012, According to New Report by Global Industry Analysts 2Global Agricultural and Environmental Diagnostics Market to Reach $2.4 Billion by 2012, According to New Report by Global Industry Analysts 3Dorset County Hospital Enhances MRSA Surveillance Programme Through Rapid Patient Screening 2Dorset County Hospital Enhances MRSA Surveillance Programme Through Rapid Patient Screening 3
Goat polyclonal to T7 tag (Agarose) Immunogen: Goats were immunized with MASMTGGQQMG (T7) conjugated to KLH.After multiple immunizations in Freund's adjuvant, serum was collected....
Insect Cell Lysis Buffer 50 ml...
Mouse monoclonal [11C9] to Zeralenone ( Abpromise for all tested applications). Antigen: Chemical / Small Molecule conjugated to BSA: Zeralenone...
Fas-ligand rabbit polyclonal antibody...
Biology Products: