Navigation Links
9 and 60 ways of particle tracking
Date:1/22/2014

A contest for the best technique of intracellular particle tracking (simultaneous tracking of the motions of hundreds and thousands of intracellular organelles, virions and even individual molecules), that is an important issue in cellular biology and has applications in search for appropriate medicines against certain diseases (including Parkinson's and Alzheimer's diseases), has found no undeniable winner. Techniques proposed by all the participants, including the Lomonosov Moscow State University professor Yannis Kalaidzidis, find their own ways for solving the problem.

The article published in Nature Methods describes not a scientific invention or discovery but the results of a contest of scientific techniques. The task was to find an original solution for a certain scientific problem, and as much as 14 groups from all over the world have taken part. Participants are co-authors of this article, while its leading authors are the organizers. Paper preparation took more than a year: it was accepted in December 2013. The problem itself was formulated in May 2012 in Barcelona during the IEEE International Symposium on Biomedical Imaging (ISBI'12).

"In the live cell imaging there is a not yet completely solved problem of simultaneous tracking of hundreds to thousands of intra-cellular organelles, vesicles, virions and individual fluorescent molecules," - explains one of the participants of the contest, Yannis Kalaidzidis, professor of the Faculty of Bioengineering and Bioinformatics, MSU and research scientist at Max Planck Institute of Molecular Cell Biology and Genetics, Dresden.

Inside the cells, there are numerous vesicles (small intracellular formations, sort of membrane-protected containers inside which nutrients and signaling molecules are stored and transported; viruses and mycobacteria also hijack them to penetrate the cell) responsible for intracellular transport, and disorders of this vesicular transport may cause a vast variety of diseases. Apart from metabolic failures, these include neurodegenerative diseases such as Alzheimer's and Parkinson's diseases and many senile genetic disorders.

In order to trace this transport, vesicles are marked and imaged by time-lapse microscopy. Previously, tracking was preformed by manual connection of vesicles on sequential frames, but today, since hundreds and thousands rather than tens of objects are tracked, manual tracking becomes problematic. Such an extensive study requires computer-based analysis. There are number of numerical algorithms developed for the tracking and practically every research group use its own. To compare the efficiencies of different methods, organizers used artificially generated time series of images where the participants had to track all the particles. Reconstructed particle trajectories were then compared to the real trajectories used during the simulations.

The contest actually ended with no single winner. The problem was equally well resolved by different techniques, meaning that, as in Kipling's verse, "There are nine and sixty ways of constructing tribal lays, And every single one of them is right!" ("In the Neolithic Age", Stanza 5, 1895). To be more precise, in this particular case every single one is equally right.

According to Kalaidzidis who presented on the contest his program MotionTracking developed for tracking of intracellular organelles in 2001-2004 and described in a 2005 paper, the negative result of the competition was predetermined by the ill-posedness and complexity of the problem. The drawback of the problem formulation that should be corrected in future contests is over-simplification of the test images that makes the problem solvable for algorithms that may fail when confronted with real data. At the same time, organizers note that the inability to track particles without errors even in such simplified image series means the problem remains unsolved and requires more profound analysis.

"This simplified approach, -- says Kalaidzidis, -- underestimates the influence of some problems present in applied quantitative microscopy and thus may be biased toward algorithms designed specifically for the contest."


'/>"/>
Contact: Ilya Usov
science-release@rector.msu.ru
Lomonosov Moscow State University
Source:Eurekalert

Related biology news :

1. Gold nanoparticles help to develop a new method for tracking viruses
2. Weighing particles at the attogram scale
3. A magnetic nanoparticles-based method for DNA extraction from the saliva after stroke
4. Oregon scientists offer new insights on controlling nanoparticle stability
5. Thousands of new particles in workplaces despite large knowledge gap
6. Ultrasound, nanoparticles may help diabetics avoid the needle
7. Nanoparticles can overcome drug resistance in breast cancer cells
8. Making complex nanoparticles easily reproducible
9. Gold nanoparticles give an edge in recycling CO2
10. Stealth nanoparticles lower drug-resistant tumors defenses
11. Researchers figure out why gold nanoparticles can penetrate cell walls
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:8/23/2017)... help is being enlisted in what,s thought to be the biggest study ... body –and are believed to affect health.  ... The Microbiome Immunity Project is the largest study to date of ... goal is to help advance scientific knowledge of the role of these ... The Microbiome ...
(Date:6/23/2017)... and ITHACA, N.Y. , June 23, ... University, a leader in dairy research, today announced a ... to help reduce the chances that the global milk ... of this dairy project, Cornell University has become the ... the Food Supply Chain, a food safety initiative that ...
(Date:5/16/2017)... , May 16, 2017   Bridge Patient ... organizations, and MD EMR Systems , an ... partner for GE, have established a partnership to ... product and the GE Centricity™ products, including Centricity ... These new integrations will allow ...
Breaking Biology News(10 mins):
(Date:10/10/2017)... ... October 10, 2017 , ... ... company advancing targeted antibody-drug conjugate (ADC) therapeutics, today confirmed licensing rights that ... Polymerized Liposomal Nanoparticle), a technology developed in collaboration with Children’s Hospital Los ...
(Date:10/10/2017)... 2017 SomaGenics announced the receipt of a ... RealSeq®-SC (Single Cell), expected to be the first commercially ... microRNAs) from single cells using NGS methods. The NIH,s ... accelerate development of approaches to analyze the heterogeneity of ... techniques for measuring levels of mRNAs in individual cells ...
(Date:10/9/2017)... , ... October 09, 2017 , ... The award-winning American ... broadcast first quarter 2018. American Farmer airs Tuesdays at 8:30aET on RFD-TV. , ... faced with the challenge of how to continue to feed a growing nation. At ...
(Date:10/7/2017)... ... October 06, 2017 , ... ... consulting for microscopy and surface analysis, Nanoscience Instruments is now expanding into ... a broad range of contract analysis services for advanced applications. Services will ...
Breaking Biology Technology: