Navigation Links
3-D tracking of single molecules inside cells
Date:3/8/2011

WASHINGTON, D.C. (March 8, 2011) -- Researchers at the University of Texas Southwestern Medical Center and the University of Texas at Dallas are reporting today at the 55th Annual Biophysical Society Annual Meeting in Baltimore, MD how they are using a novel 3D cell imaging method for studying the complex spatial-temporal dynamics of protein transport, providing a solution to this fundamental problem in cell biology.

According to the authors of the study, imaging such highly dynamic processes in the cell and in 3D poses major technical challenges in a complex cell monolayer due to cell-to-cell variations in thickness and temporal properties of protein transport. Previous imaging techniques were slow and suffered from poor z-localization/3D-tracking capability.

Using a combination of multifocal plane microscopy (MUM) and nanodot labeling technology, the researchers were able to label single molecules in live cells and track their movement and their interaction with other molecules in a thick cell sample for extended periods of time.

Sripad Ram, the lead author of the study, explains that the main reason he and his colleagues developed these imaging techniques is to track the movement of therapeutic antibodies, which are engineered in their lab. "We want to know where these antibodies go and what they do once they enter the body," says Ram.

He adds that "current microscopy technologies are limited in that you can only image a single focal plane at any given time. If you want to image in three dimensions, you can only do so sequentially, but you end up imaging at the wrong place and at the wrong time thereby missing events over time.what we needed was a technology that could simultaneously image a sample across multiple planes, and that is what multifocal plane microscopy is all about."


'/>"/>

Contact: Ellen R. Weiss
eweiss@biophysics.org
240-290-5606
American Institute of Physics
Source:Eurekalert

Page: 1

Related biology news :

1. Tracking forest threats
2. Shark tracking reveals impressive feats of navigation
3. Tests show bright future for gadonanotubes in stem cell tracking
4. Tracking $40 billion in commitments for maternal and child health
5. Online Publishers Association Unveils Biometric and Eye Tracking Research to Assess the Effectiveness of New OPA Ad Units
6. Tracking down pathogenic yeasts
7. Tracking triclosans field footprint
8. Census of Marine Life program tracking animals on Pacific continental shelf
9. Electronic tracking system can improve follow-up after an abnormal Pap test
10. Nanotech medicine, tumor tracking, new technologies and more
11. Genome signatures enable tracking of algal complexity
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:4/26/2016)... -- Research and Markets has announced the ...  report to their offering.  , ,     (Logo: ... forecast the global multimodal biometrics market to grow ... 2016-2020.  Multimodal biometrics is being implemented ... healthcare, BFSI, transportation, automotive, and government for controlling ...
(Date:4/14/2016)... , April 14, 2016 ... and Malware Detection, today announced the appointment of ... the new role. Goldwerger,s leadership appointment comes ... the heels of the deployment of its platform at ... behavioral biometric technology, which discerns unique cognitive and physiological ...
(Date:3/29/2016)... LegacyXChange, Inc. (OTC: LEGX ... Protect are pleased to announce our successful effort to ... of writing instruments, ensuring athletes signatures against counterfeiting and ... athletes on LegacyXChange will be assured of ongoing proof ... Bill Bollander , CEO states, "By inserting ...
Breaking Biology News(10 mins):
(Date:6/23/2016)... Calif. , June 23, 2016  Blueprint Bio, ... biological discoveries to the medical community, has closed its ... Matthew Nunez . "We have received ... with the capital we need to meet our current ... essentially provide us the runway to complete validation on ...
(Date:6/23/2016)... ... June 23, 2016 , ... ClinCapture, the only free ... and will showcase its product’s latest features from June 26 to June 30, ... poster on Disrupting Clinical Trials in The Cloud during the conference. DIA ...
(Date:6/23/2016)... FRANCISCO , June 22, 2016  Amgen (NASDAQ: ... sponsorship of the QB3@953 life sciences incubator ... human health. The shared laboratory space at QB3@953 was ... overcome a key obstacle for many early stage organizations ... part of the sponsorship, Amgen launched two "Amgen Golden ...
(Date:6/22/2016)... , June 22, 2016 Research and ... Global Markets" report to their offering. ... billion in 2014 from $29.3 billion in 2013. The market is ... of 13.8% from 2015 to 2020, increasing from $50.6 billion in ... projected product forecasts during the forecast period (2015 to 2020) are ...
Breaking Biology Technology: