Navigation Links
3-D 'map' of enzyme completed by MU scientists could lead to more effective drugs
Date:5/13/2014

COLUMBIA, Mo. The human body is full of proteins called enzymes that help nearly every function in the body. Scientists have been studying enzymes for decades in order to learn how they work and how to create better drugs and medical treatments for many ailments. Now, University of Missouri researchers have completed a 3-D map of an enzyme called Proline utilization A (PutA). PutA facilitates metabolism by adding oxygen to molecules. John Tanner, a professor in the MU Department of Biochemistry, says mapping this enzyme will give researchers a better understanding of its function, which could help drug manufacturers create more effective drugs.

"PutA is actually two enzymes fused together to make its processing more efficient," Tanner said. "Now that we have an understanding about how PutA is constructed, we can study exactly how it works. Some dangerous bacteria, such as h. pylori, which infect stomach tissue, utilize the PutA enzyme to grow. Discovering the structure of this enzyme will provide valuable insight into how this protein functions and could provide blueprints for designing drugs that inhibit or increase certain protein functions, which would make those drugs more effective."

Tanner conducted his study by using a process called protein crystallography. This process involves growing microscopic crystals made of PutA enzymes. Tanner then shipped the protein crystals to Berkeley, Calif., where they were exposed to a high-powered X-ray device called a "beamline." (The University of Missouri is a founding member of the Molecular Biology Consortium (MBC), which operates the beamline in California.) The beamline device is one of only five in the world and is the size of a football field.

The beamline captures X-rays, focuses them on protein crystals, and records the beams that reflect or "diffract" off the crystals. Tanner decoded the diffraction patterns from his crystals to understand the precise arrangement of the atoms in the protein and created a 3-D "map" of the PutA enzyme.

"This entire project would not be possible without our access to the MBC beamline," Tanner said. "The X-ray device we have on campus has only a fraction of the power of the beamline, and we would not be able to complete this complex mapping project without such a high-powered X-ray device."

Tanner says the next step in his research is to explore the functions of each individual structure of the PutA enzyme to better understand how the enzyme works. The Department of Biochemistry is in the MU College of Food, Agriculture and Natural Resources and the MU School of Medicine. Tanner is also a professor in the Department of Chemistry in the MU College of Arts and Science.


'/>"/>

Contact: Nathan Hurst
hurstn@missouri.edu
573-882-6217
University of Missouri-Columbia
Source:Eurekalert  

Related biology news :

1. Mapping the spider genome
2. Putting the endoparasitic plants Apodanthaceae on the map
3. Norovirus in food outlets to be mapped for the first time
4. Finding the switch: Researchers create roadmap for gene expression
5. Researchers present comprehensive roadmap of blood cells
6. Stanford professor maps by-catch as unintended consequence of global fisheries
7. Change happens: New maps reveal land cover change over 5 years across North America
8. 3D scans map widespread fish disease
9. A road map -- and dictionary -- for the arthropod brain
10. Maps show expected redistribution of global species due to climate change
11. Where do lizards in Qatar live? First distribution maps for the state
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
3-D 'map' of enzyme completed by MU scientists could lead to more effective drugs
(Date:2/16/2017)... , Feb. 16, 2017  Genos, a ... announced that it has received Laboratory Accreditation from ... Accreditation is presented to laboratories that meet stringent ... demonstrate scientifically rigorous processes. "Genos is ... in laboratory practices. We,re honored to be receiving ...
(Date:2/14/2017)... 2017  Wake Forest Baptist Medical Center today announced Julie ... executive officer (CEO). Freischlag joins the medical center on ... , M.D., who last year announced that he would ... after leading it since 2008.   As ... Forest Baptist,s academic health system, which includes Wake Forest ...
(Date:2/13/2017)... SAN FRANCISCO , Feb. 13, 2017 /PRNewswire/ ... a centralized platform that is designed to enhance ... the latest release in the RSA Fraud & ... to enable organizations to leverage additional insights from ... anti-fraud tools to better protect their customers from ...
Breaking Biology News(10 mins):
(Date:2/16/2017)... Feb. 16, 2017  MDNA Life Sciences Inc. ... of liquid biopsy tests based on the mitochondrial ... exclusive license agreement with its first international commercial ... biopsy test for prostate cancer, the Prostate Mitomic ... . This is the first overseas appointment for ...
(Date:2/16/2017)... 2017 Patient Care services ... telemedicine application, new and leading edge therapies and ... boom worldwide. The healthcare sector as whole continues ... and new therapies for companies such as Reliq ... Biosciences, Inc. (NASDAQ: CLRB ), Cytori ...
(Date:2/16/2017)... Pa. , Feb. 16, 2017  Windtree ... biotechnology company focusing on developing aerosolized KL4 surfactant ... from a preclinical influenza study showed that aerosolized ... survival in a well-established preclinical animal model. The ... a growing body of evidence that supports the ...
(Date:2/15/2017)... ... ... Park Systems , a leader in Atomic Force Microscopy (AFM) since ... productivity with single click reliable nanoscale images, is now available on Park XE series ... the image once done manually by the operator producing high quality nanoscale imaging in ...
Breaking Biology Technology: