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3-D long-term bone marrow culture to analyze stromal cell biological function
Date:11/1/2011

oblast cell line (MC3T3E1 cell) and chondrocyte cell line (ch-8 cell), can adhere easily on the G-02 particle and proliferate rapidly on its surface. This advantage of the G-02 particle enables us to develop 3D BMCS, which can also be applied to other 3D organ cultures of central nerve system, heart and liver."

CD34 is well known to be a surface marker for human primitive hematopoietic progenitor cells, however, CD34+ cells can also differentiate into stromal cells. When CD34+ cells are co-cultured with human stromal cells, it is complex to clarify the biological function of the preestablished stromal cell layer. Prof. Shin Aizawa, group leader and pioneer in the study of stromal cell, says "In this study, we used a murine stromal cell line (MS-5) instead of human stromal cells to exclude the effect of CD34+ derived stromal cells. This co-culture system makes it possible to distinguish the function of MS-5 stromal-cell layer from that of CD34+ derived stromal cells. Now our group is studying gene-expression levels of various cytokines in stromal cells using specific primers and probes for the mouse and human".

Steven R. Goodman, Ph.D. Editor-in-Chief of Experimental Biology and Medicine said "this 3-dimensional bone marrow culture system, developed by Hirabayashi and coworkers, should be an outstanding tool for the study stromal cell function".


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Contact: Dr. Tomonori Harada
harada.tomonori@nihon-u.ac.jp
Society for Experimental Biology and Medicine
Source:Eurekalert

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