Navigation Links
3-D long-term bone marrow culture to analyze stromal cell biological function
Date:11/1/2011

Stromal cells, as distinct from hematopoietic cells, are an essential component of the bone marrow microenvironment and are necessary for the long-term maintenance of hematopoietic stem cells (HSCs) in vitro. Previous studies have shown that stromal cells regulate the proliferation and differentiation of HSCs through the production of diffusible hematopoietic regulatory factors and extracellular matrix, and through physical cell-cell interactions involving adhesion molecules and gap junction-mediated cell communication. However, the ability of stromal cells to support the expansion of HSCs and to maintain their self-renewal potential has generally been investigated in long-term, two-dimensional (2D) bone marrow culture systems (BMCS), and most of the reports have shown a decline in HSCs within 4𔃆 weeks in culture.

In work published in the November 2011 issue of Experimental Biology and Medicine, Hirabayashi and co-investigators from Nihon University School of Medicine, Osaka Prefecture University and the Norwegian University of Science and Technology have developed a new three-dimensional (3D) BMCS. As stated by co-author Isao Tsuboi, "2D BMCS can't maintain HSCs for a long time, which does not enable us to analyze stromal-cell function. Therefore, we developed a new 3D BMCS and succeeded to maintain HSCs for much longer time."

This new 3D BMCS is based on unique particles. As explained by co-author Yukio Hirabayashi, "The polymer particle with grafted epoxy-polymer-chains is most important in our 3D BMCS. We selected the particle most suitable for cell culture from more than 20 types of particles with various grafted polymer chain lengths and its surface density, the composition of base polymer network and graft polymer chain. We named this particle G-02". Furthermore, co-author Tomonori Harada adds "Several kinds of cell lines, other than murine fibroblast cell line (MS-5 cell), such as an epidermal cell line (HeLa cell), osteoblast cell line (MC3T3E1 cell) and chondrocyte cell line (ch-8 cell), can adhere easily on the G-02 particle and proliferate rapidly on its surface. This advantage of the G-02 particle enables us to develop 3D BMCS, which can also be applied to other 3D organ cultures of central nerve system, heart and liver."

CD34 is well known to be a surface marker for human primitive hematopoietic progenitor cells, however, CD34+ cells can also differentiate into stromal cells. When CD34+ cells are co-cultured with human stromal cells, it is complex to clarify the biological function of the preestablished stromal cell layer. Prof. Shin Aizawa, group leader and pioneer in the study of stromal cell, says "In this study, we used a murine stromal cell line (MS-5) instead of human stromal cells to exclude the effect of CD34+ derived stromal cells. This co-culture system makes it possible to distinguish the function of MS-5 stromal-cell layer from that of CD34+ derived stromal cells. Now our group is studying gene-expression levels of various cytokines in stromal cells using specific primers and probes for the mouse and human".

Steven R. Goodman, Ph.D. Editor-in-Chief of Experimental Biology and Medicine said "this 3-dimensional bone marrow culture system, developed by Hirabayashi and coworkers, should be an outstanding tool for the study stromal cell function".


'/>"/>

Contact: Dr. Tomonori Harada
harada.tomonori@nihon-u.ac.jp
Society for Experimental Biology and Medicine
Source:Eurekalert

Related biology news :

1. March of Dimes honors researcher who linked maternal obesity to a babys long-term health
2. Glucocorticoid treatment may prevent long-term damage to joints
3. Earth: 9/11s lingering effects: Tracking the long-term impact of the dust
4. Plenary speakers address challenges in the delivery of sustained antiretroviral therapy in developing countries, call for social scientists to take their place at the HIV/AIDS policy-making table, and stress the need for a long-term response to AIDS
5. LSUHSC conducts landmark study of mid, long-term health effects of oil exposure
6. The long-term fiscal impact of funding cuts for IVF in Denmark
7. Targeted adalimumab treatment can optimize long-term outcomes for patients with early RA
8. Scripps Research scientists identify mechanism of long-term memory
9. Louisiana, Florida residents differ on views of long-term effects of oil spill
10. Long-term methadone treatment can affect the brain
11. Scientists discover major clue in long-term memory making
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:2/16/2017)... 2017  Genos, a community for personal genetic ... received Laboratory Accreditation from the College of American ... laboratories that meet stringent requirements around quality, accuracy ... "Genos is committed to maintaining the ... honored to be receiving CAP accreditation," said ...
(Date:2/13/2017)... SAN FRANCISCO , Feb. 13, 2017 /PRNewswire/ ... a centralized platform that is designed to enhance ... the latest release in the RSA Fraud & ... to enable organizations to leverage additional insights from ... anti-fraud tools to better protect their customers from ...
(Date:2/10/2017)... Research and Markets has announced the addition of ... Commercial Aspects" to their offering. ... Biomarkers play an important ... selection of treatment as well for monitoring the results. There ... modern medicine. Biochip/microarray technologies and next generation sequencing are also ...
Breaking Biology News(10 mins):
(Date:3/28/2017)... -- Summary This report provides all the ... partnering interests and activities since 2010. Description The Partnering ... into the partnering activity of one of the world,s leading ... prepared upon purchase to ensure inclusion of the most up ... will be delivered in PDF format within 1 working day ...
(Date:3/28/2017)... March 28, 2017  Viking Therapeutics, Inc. ("Viking") (NASDAQ: ... of novel therapies for metabolic and endocrine disorders, today ... , Ph.D., will deliver a corporate presentation at H.C. ... Conference, being held April 3, 2017 at the St. ... for this presentation are as follows: ...
(Date:3/28/2017)... ... March 28, 2017 , ... Currently symptomatic therapies for ... recent study published in STEM CELLS suggests that human neural stem cell (hNSC) ... to produce more neural cells. , Strategies involving transplantation of these cells ...
(Date:3/28/2017)... Triangle Park, NC (PRWEB) , ... March 28, ... ... development company engaged in the development of a new orally administered treatment for ... Advisory Board. , CEO John Didsbury states, “As we seek to uniquely treat ...
Breaking Biology Technology: