Transcriptase

reverse transcriptase
the enzyme that synthesizes DNA from an RNA intermediate
Source: Jenkins, John B. 1990. Human Genetics, 2nd Edition. New York: Harper & Row ...
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Reverse Transcriptase
The enzyme that catalyzes the reverse transcription from RNA to DNA.
Related ...
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Reverse Transcriptase Polymerase Chain Reaction
A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The cDNA is then amplified using standard PCR protocols.
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z ...
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Reverse Transcriptase Inhibitors
Protease Inhibitors
Fusion Inhibitors
Integrase Inhibitors
Inhibiting Coreceptor Binding ...
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Reverse transcriptase
An enzyme that catalyzes the synthesis of DNA from an RNA template.
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Reverse transcriptase An enzyme used by retroviruses to form a complementary DNA sequence (cDNA) from their RNA. The resulting DNA is then inserted into the chromosome of the host cell.
Ribonucleotide See: nucleotide ...
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reverse transcriptase
(trans-krip-tase)
An enzyme encoded by some RNA viruses that uses RNA as a template for DNA synthesis.
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Reverse transcriptase
An enzyme produced by retroviruses that can synthesize a strand of DNA complimentary to an RNA template. (Reverse transcriptase can also synthesize DNA from a DNA template.) Used to make cDNA clones from mRNA.
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REVERSE TRANSCRIPTASE - A DNA polymerase which copies an RNA molecule into single-stranded cDNA; usually purified from retroviruses.
REVERSE TRANSCRIPTION - Copying of an RNA molecule into a DNA molecule.
REVERTANT - See BACK MUTATION.
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Reverse transcriptase (RNA-dependent DNA polymerase). An enzyme isolated from retrovirus-infected cells that synthesizes a complementary (c)DNA strand from an RNA template.
RFLP. See Restriction-fragment-length polymorphism.
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Reverse transcriptase: An enzyme which will make a DNA copy of an RNA template - a DNA-dependant RNA polymerase.
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Reverse transcriptase: An enzyme found in the retroviruses which catalyzes the RNA-dependent polymerization of DNA (DNA copy from RNA template).
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Use reverse transcriptase (from retroviruses, see discussion above) to produce a DNA copy of the RNA. This copy is called cDNA.
Expression Vectors ...
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DNA synthesized from an RNA template using reverse transcriptase. cDNA library. A library composed of complementary copies of cellular mRNAs. (See Library.) Cellular oncogene (proto-oncogene).
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cDNA (complementary DNA) A DNA, usually made by reverse transcriptase, which is complementary to given mRNA and used in cloning. central dogma The organizing principle of molecular biology: genetic information flows from DNA to RNA to protein.
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Several anti-retroviral drugs work by interfering with reverse transcriptase, the key enzyme of retroviruses. These drugs, the reverse transcriptase inhibitors, act when cells first become infected.
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Retroviruses, such as Human Immunodifficiency Virus (HIV), also include the enzyme reverse transcriptase with the viral RNA. Reverse transcriptase makes a single-stranded viral DNA copy of the single-stranded viral RNA.
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Negative-sense viruses must have their genome copied by a RNA polymerase or transcriptase to form mRNA, which is then translated into proteins by the host. Like the above method, RNA replicase is used to produce new virions.
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retrotransposon - A transposable DNA element (transposon) which is replicated through an RNA intermediate via reverse transcriptase. Within their characteristic long terminal repeats (LTRs), some retrotransposons encode retroviral-like proteins (e.g.
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While transcription was classically thought to only occur from DNA to RNA, reverse transcriptase transcribes RNA into DNA. The term "retro" in retrovirus refers to this reversal of the Central dogma of molecular biology.
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The pol-like open reading frame is probably the gene for a reverse transcriptase that transcribes processed RNA into DNA.
Elements such as these retrotransposons or retroposons are derived from or are the source of retroviruses.
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(cDNA): Single-stranded DNA produced from an RNA template (usually mRNA) by
reverse transcriptase
in vitro. It lacks the introns present in corresponding genomic DNA. It is most commonly made to use in PCR to amplify RNA (
RT
-PCR).
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Certain viruses have the ability to insert a copy of themselves into the genome of a host. The chemical that make this possible (reverse transcriptase) is widely used in genetic engineering.
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complementary DNA (cDNA) DNA prepared by transcribing the base sequence from mRNA into DNA by reverse transcriptase; also called copy DNA.
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RT-PCR PCR amplification from an RNA template. The first step involves synthesis of a single strand of cDNA on the RNA template using reverse transcriptase. The cDNA is then used as the template for PCR to produce a DNA product.
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reverse transcriptase viral enzyme used to make cDNA. RFLP -- restriction fragment length polymorphism; variations occurring within a species in the length of DNA fragments generated by a species endonuclease.
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