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Stringency


Stringency
Hybridization conditions: higher the stringency, lower the probability of hybridization. Increase the temperature or decreasing the salt concentration raises the stringency.
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Stringency
Conditions affecting the hybridization of nucleotide sequences. Higher stringency conditions require more base pairing between the two sequences.
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STRINGENCY - The conditions employed for hybridization which determine the specificity of the annealing reaction between two single-stranded nucleic acid molecules.
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Stringency: A term used to describe the conditions of hybridization. By varying the conditions (especially salt concentration and temperature) a given probe sequence may be allowed to hybridize only with its exact complement (high stringency), ...
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These issues include relative quantitation by low-stringency PCR, the Cot effect, and problems of target vs standard titration.
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