Sticky ends

Sticky ends
Digestions of double-stranded DNA by many restriction enzymes (e.g. EcoR I) generate ends with a short single-stranded sequences. Such ends are called sticky ends.
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sticky ends Term applied to DNA sequences cut with restriction enzymes where the cuts will bond with each other or with another sequence cut with the same enzyme.
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Sticky ends: After digestion of a DNA with certain restriction enzymes, the ends left have one strand overhanging the other to form a short (typically 4 nt) single-stranded segment.
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DNA insert and vector molecules are digested with two different restriction enzymes to create noncomplementary sticky ends at either end of each restriction fragment.
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These are called "sticky ends" because they are able to base pair with any DNA molecule containing the complementary sticky end.
In this case, both DNA preparations have complementary sticky ends and thus can pair with each other when mixed.
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Transposase makes a staggered cut at the target site producing sticky ends, cuts out the transposon and ligates it into the target site.
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Fragments of DNA that has been cut with restriction enzymes have unpaired nucleotides at the ends called sticky ends. All of the fragments will have the same sticky ends. The sticky ends have complimentary bases, so they could rejoin.
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Most enzymes make slightly staggered incisions, resulting in "sticky ends", out of which one strand protrudes. There are three known evolutionary lineages of restriction enzyme, which each cleave DNA by a different mechanism.
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Linker
A synthetic, double-stranded oligonucleotide used to attach sticky ends to a blunt-ended molecule.
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palindromic sequence (recognition sequence) within a double-stranded DNA molecule and then catalyzes the cleavage of both strands at that site. Also called a restriction enzyme. Restriction endonucleases may generate either blunt or sticky ends at ...
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Some restriction enzymes generate blunt ends, cutting across both strands of DNA. Others generate a staggered cut, producing "sticky ends.
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cohesive ends Two DNA ends in the same DNA molecule, or in different molecules, with short overhanging single-stranded segments that are complementary to one another, facilitating ligation of the ends; also known as sticky ends.
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