Reverse transcription

Reverse transcription
The process of copying information found in RNA into DNA catalysed by a RNA dependant DNA polymerase (reverse transcriptase). Reversal of transcription.
Related Terms: Ribonucleic acid (RNA) ...
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reverse transcription Process of transcribing a single-stranded DNA from a single-stranded RNA (the reverse of transcription); used by retroviruses as well as in biotechnology.
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Reverse transcription
Scheme of the Reverse transcription
Some viruses have the ability to transcribe RNA into DNA in order to infect a cell's genome. The main enzyme responsible for this type of transcription is called reverse transcriptase.
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Reverse transcription (RNA → DNA) lacks the proofreading capabilities of DNA replication or of normal transcription (DNA → RNA). Therefore errors, i.e., mutations, are frequent. Because of these, ...
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REVERSE TRANSCRIPTION - Copying of an RNA molecule into a DNA molecule.
REVERTANT - See BACK MUTATION.
RIBONUCLEASE (RNase) - An enzyme which catalyzes the hydrolysis of RNA. There are many different RNases, some of the more important include: ...
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Because reverse transcription is missing the usual "proofreading" of DNA transcription, this kind of virus mutates very often.
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RT See Reverse transcription (RT)
RT-PCR See Reverse Transcription-PCR (RT-PCR)
Recombinant
Recombinant DNA (rDNA)
Recombination frequency
Replication fork
Response element
Restriction
Restriction Fragment Length Polymorphism (RFLP) ...
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A technique involving reverse transcription of purified mRNA into the corresponding complementary DNA prior to insertion into a vector.
Cell extract
A preparation consisting of a large number of broken cells and their released contents.
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A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The cDNA is then amplified using standard PCR protocols.
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z ...
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Because they use RNA, their cellular hosts may synthesize their proteins as soon as they are infected and without the delay in waiting for transcription. On the other hand, RNA retroviruses, such as AIDS, require the reverse transcription of their ...
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However, because the initial reverse transcription is often shortened, cDNA libraries with abundant truncated products are the common result, particularly for the longer source mRNAs.
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