Reporter Gene

A gene whose phenotypic expression is easy to monitor; used to study promoter activity in different tissues or developmental stages. Recombinant DNA constructs are made in which the reporter gene is attached to a promoter region of particular ...
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Reporter Genes
With gene splicing, the promoter of a gene whose expression you wish to monitor can be coupled to the coding sequence of a chosen "reporter" gene.
lacZ ...
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Reporter gene See: marker
Resolution Degree of molecular detail on a physical map of DNA, ranging from low to high.
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Reporter gene
A gene which can be placed downstream of a promoter and expression of the gene followed by a relatively easy assay (often a colorimetric assay). See operon and gene fusions.
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Reporter gene Gene coding for an easily assayed protein which is used to detect expression of the gene under different conditions; usually to test the activity of a promoter.
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Reporter Gene: The use of a functional enzyme, such as beta-galactosidase, luciferase, or chloramphenicol acetyltransderase, downstream of a gene, promoter, or translational control element of interest, ...
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Reporter genes inserted at any of these loci are not transcribed as well. The main effector of silencing at these loci is the SIR complex.
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So we used a technology called the "reporter gene." Reporter gene is simply a device that you can rig up (because of the technology that we have available today) so that when the cells fuse they turn on a gene that we can easily measure.
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(CAT): The bacterial gene for chloramphenicol, CAT, is commonly used as a reporter gene for investigating physiological gene regulation. Beta-
galactosidase
and luciferase genes can also be used for the same purpose.
Chloroplast ...
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Chloramphenicol Acetyl Transferase (CAT) is a bacterial enzyme which inactivates chloramphenicol by acetylating it. Used as a reporter gene for promoter studies.
Related
GFP Luciferase assay ...
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Bacterial genes have been inserted into other organisms as reporter genes.
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It is easier to perform a CAT assay than it is to do a Northern blot, so CAT assays were a common method for testing the effects of sequence changes on promoter function. Largely supplanted by the reporter gene luciferase.
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