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Relaxed DNA


In a "relaxed" double-helical segment of DNA, the two strands twist around the helical axis once every 10.4-10.5 base pairs of sequence. Adding or subtracting twists, as some enzymes can do, imposes strain. If a DNA segment...
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... twisting it about the other strand, to relax DNA to a thermodynamic equilibrium. Once DNA is relaxed, topoisomerase reconnects broken strands. Structure ...
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... with the amount of relaxed DNA to get you going and ... Relaxed, modified pBR-322 1000 ug -High purity DNA gyrase holoenzyme 2000 units -Assay buffer ...
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... enzyme with the amount of relaxed DNA to get you going and ... Relaxed, modified pBR-322 500 ug -High purity DNA gyrase holoenzyme 1000 units -Assay buffer ...
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Relaxation of DNA-loops was analysed by the method of anomalous viscosity time ... The dose-responses for DNA-loop relaxation, induction of at least one residual ...
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All topoisomerases can relax supercoiled DNA, and DNA gyrase, present in ... the inter-conversion of relaxed and supercoiled DNA, is not readily monitored. ...
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... way to estimate the relaxation time of DNA in varying degrees of ... relaxation times of DNA were extracted ... The relaxation behavior of DNA confined in a ...
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Any deviation from this relaxed state increases the energy of the DNA molecule. ... Relaxed DNA is in the "B" configuration, with 10 base pairs per helical turn. ...
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DNA in its relaxed (ideal) state usually assumes the B configuration, a right ... Any deviation from this relaxed state increases the energy of the DNA molecule. ...
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DNA in its relaxed (ideal) state usually assumes the B configuration. In a relaxed double-helical segment of DNA, the two strands twist around the ...
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The active DNA inside a cell is not relaxed DNA, but instead ... DNA is said to be relaxed (FIGURE ... For the relaxed DNA shown in FIGURE 44), the ...
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The DNA relaxation function of TOP1 is essential for the fundamental biological ... The supercoiled and relaxed DNA bands are shown. ...
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overwound compared with the relaxed DNA. ... in shape between supercoiled and relaxed DNA molecules. ... relaxed intracellular DNA, which is usually achieved ...
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This loss of superhelical stress puts the plasmid into a "relaxed DNA" form. ... micrograph: This one is of relaxed DNA. Relaxed DNA (Bock lab) What is a ...
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... to Relaxed and Linear Forms in Nucleobond AX Plasmid DNA Preparations ... DNA using the Nucleobond AX alkaline lysis-based protocol, occasionally relaxed ...
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DNA relaxation assay was used to characterize the purified MtTOP. ... Relaxation of negatively supercoiled DNA to determine unit activity. ...
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... Navarra, the Polytechnic University of Madrid (CBGP), the University ... Institute of Agricultural Research have managed to sequence the ... olive tree. The study, included in the June issue ... of the genome of a pathogenic bacteria undertaken in ...
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