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Primer extension


PRIMER EXTENSION - A reaction in which DNA is reverse transcribed from an RNA template to which a specific oligonucleotide primer has been annealed.
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Primer extension: This is a method used to figure out how far upstream from a fixed site the start of an mRNA is. For example, perhaps you have isolated a cDNA clone, but you don't think that the clone has all of the 5' untranslated region.
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Singlesided primer extension with dideoxynucleotide chain terminators is avoided with the consequence that the sequencing fragments are derived directly from the original PCR products.
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The temperature is then raised again to about 72 C for primer extension.
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In these cases, although the mRNA is monocistronic, the nuclear transcript was polycistronic.
S1 nuclease mapping of 5' ends of mRNAs produced by trans-splicing, unlike primer extension, does not locate the true 5' end of the RNA.
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