Primer

Primer
A short oligonucleotide complementary to target DNA and acts as the leader for DNA extension. Also refers to short oligonucleotide used to primer reverse transcription of RNA to DNA in reverse transcription reactions. See PCR Glossary.
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Primer design
The choice of the length of the primers and their melting temperature depends on a number of considerations.
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Primer on Molecular Genetics
from the U.S. Department of Energy
This primer was prepared by Denise Casey, Human Genome Management Information System, Oak Ridge National Laboratory, for the 1991-92 DOE Human Genome Program Report.
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Definition of primer :
A short sequence (of RNA or DNA) from which DNA replication can initiate. May be either a synthetic DNA or RNA or a length of RNA synthesized in vivo by primase.
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Primer
This article is about molecular biology for alternate uses, see primer (disambiguation).
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Dr. John Carpten, of the National Human Genome Research Institute's Laboratory of Cancer Genetics, defines primer.
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terms:
polymerase chain reaction (PCR) ...
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Primer
Short preexisting single-stranded polynucleotide chain to which new deoxyribonucleotides can be added by DNA polymerase. It anneals to a nucleic acid template and promotes copying of the template starting from the primer site.
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Primer pheromones are detected by the olfactory epithelium with which normal odors are detected and also in most mammals (but not humans) by the vomeronasal organ (VNO).
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Primer
A short oligonucleotide complementary to a strand of DNA or RNA that is used to initiate synthesis of the complementary DNA strand.
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Primers are short chains of about 20 nucleotides that are complimentary to a region in the DNA to be amplified. They are needed because the enzyme that copies the DNA (DNA polymerase) cannot start the process unless it has already been started.
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primer
An already existing short RNA chain bound to template DNA to which DNA nucleotides are added during DNA synthesis.
primate ...
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PRIMER - An oligonucleotide which is complementary to a specific region within a DNA or RNA molecule, and which is used to prime (initiate) synthesis of a new strand of complementary DNA at that specific site, ...
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Primer. A short DNA or RNA fragment annealed to single-stranded DNA, from which DNA polymerase extends a new DNA strand to produce a duplex molecule.
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Primer extension: This is a method used to figure out how far upstream from a fixed site the start of an mRNA is. For example, perhaps you have isolated a cDNA clone, but you don't think that the clone has all of the 5' untranslated region.
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RNA primers - primers used to synthesize DNA strands by acting as a template
RNA splicing - process in which intron sequences are excised from RNA molecules in the nucleus during formation of messenger RNA ...
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Most often used to describe the binding of a short primer or probe. Antibiotic. A class of natural and synthetic compounds that inhibit the growth of or kill other microorganisms.
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primase An enzyme that synthesizes RNA oligonucleotides used as primers by DNA polymerases. primer A short oligomer (of sugars in glycogen or nucleotides in DNA) to which an enzyme adds additional monomeric subunits.
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In practice, when synthesising an oligonucleotide primer or probe based on this information a mixture of all the possible sequences is made, and referred to as a degenerate oligonucleotide.
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As it cools, primers bind the DNA. These primers are short oligonucleotides chosen by the experimenter and added to the DNA mixture at the beginning. They flank the region to be amplified.
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For the primers to anneal to the ssDNA fragments, the temperature is reduced to about 55 C. However, at this temperature the original complementary ssDNA fragments will begin to re-anneal with each other.
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If the region between primers A and B contains a restriction site that is polymorphic, the PCR product can be digested with the enzyme and the size of the digestion products analyzed by gel electrophoresis.
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An enzyme, requiring a DNA primer, that catalyzes the synthesis of a DNA strand from an RNA template. An enzyme that can use RNA as a template to synthesize DNA.
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z ...
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Source: The Brain: A Neuroscience Primer written by Richard F. Thompson, 3rd edition (User Submission)
What Topic Is Your BIOLOGY Paper On?
ENTER YOUR TOPIC HERE: ...
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Primer on Molecular Genetics (Department of Energy)
Tutorial on EUKARYOTIC DNA TRANSCRIPTION (UC Davis)
Glossary (DOE) Terms peculiar to molecular genetics.
Gene Zine A very good beginning on how genes work.
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Low explosive trains are something like a bullet - Primer and a propellant charge. High explosives trains can be more complex, either Two-Step (e.g. Detonator and Dynamite) or Three-Step (e.g. Detonator, Booster and ANFO).
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: A loop of nucleic acid formed by duplex formation within a single strand (also called stem loop). If happens in a PCR primer, it will not function.
Haploid number ...
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Primer -- nucleotides used in the polymerase chain reaction to initiate DNA synthesis at a particular location. Probability -- the long term frequency of an event relative to all alternative events, and usually expressed as decimal fraction.
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