Polyadenylation

Polyadenylation sites in the 3' ends of genes transcribed byRNA polymerase II will be analyzed. These sites, key signals in3' flanking regions of a given group of genes, determinefeatures of 3'-end processing of pre-mRNAs.
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CPEB ("cytoplasmic polyadenylation element binding protein") is a protein that
is found in neurons of the central nervous system (as well as elsewhere);
stimulates messenger RNA (mRNA) translation;
is needed for long-term facilitation (LTF).
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Where several alternative polyadenylation sites are found, preference is governed by a third sequence element upstream of AAUAAA.
PolyA tails are thought to function in mRNA stability and in initiation of translation.
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Alternative selection of cleavage/polyadenylation sites: this is the only method of splicing which can produce an alternative C-terminus domain in proteins. In this case, different sets of polyadenylation sites can be spliced with the other exons.
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Complexes formed by snRNA and proteins. Involved in RNA processing such as splicing and polyadenylation.
Related
Dot blot Far Western blot Northern blot Polyadenylation Splicing Western blot snRNA ...
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mRNA processing: Refers to the processes of polyadenylation, splicing, and addition of a 5' cap structure.
Metaphase: A stage in mitosis or meiosis during which the chromosomes are aligned along the equatorial plane of the cell.
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Processing: The reactions occurring in the nucleus which convert the primary RNA transcript to a mature mRNA. Processing reactions include capping, splicing and polyadenylation.
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their function nor of the genetics of the organism. This can be accomplished because different gene features, such as exons, introns, promoters, polyadenylation signal etc are associated with unique patterns in the DNA sequence.
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