PCR

PCR (in vitro DNA amplification)
Described as being to genes what Gutenberg's printing press was to the written word, ...
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A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The cDNA is then amplified using standard PCR protocols.
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PCR speeds RFLP and VNTR molecular marker analysis.
Facts
Classical RFLP analysis by Southern blotting and hybridization required the testing laboratory to have on hand DNA molecules from which to make probes.
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With PCR, it is routinely possible to amplify enough DNA from a single hair follicle for DNA typing. Some workers have successfully amplified DNA from a single sperm cell.
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A fast, inexpensive technique for making an unlimited number of copies of any piece of DNA. Sometimes called "molecular photocopying," PCR has had an immense impact on biology and medicine, especially genetic research.
explained: ...
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PCR in practice
Figure 1: PCR machine
PCR is used to amplify a short, well-defined part of a DNA strand. This can be a single gene, or just a part of a gene.
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PCR
See polymerase chain reaction.
Related Terms: Polymerase chain reaction (PCR) ...
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PCR Polymerase chain reaction. An in vitro technique to produce many copies of a specific section of DNA sequence. PCR is normally used to amplify sections up to ~2kbp in length, although routine PCR of sections up to 20kbp is becoming possible.
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PCR
Polymerase chain reaction. A method for amplifying a particular region of DNA by a sequence of denaturation, annealing of specific primers, and synthesis.
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PCR: See polymerase chain reaction.
P henotype: The outward physical characteristics of an organism.
Physical map: A map of the locations of identifiable landmarks on DNA, such as genes, or restriction enzyme cutting sites.
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PCR: polymerase chain reaction; a technique used to amplify a gene of interest.
peptides: small proteins.
peripheral nervous system: a collection of nerves that connect the brain and spinal cord to other parts of the body and the external environment.
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PCR .See polymerase chain reaction.
peanut worms Burrowing invertebrates with an unsegmented body and an anterior end that can be pulled into the body.
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PCR is a technique that allows a researcher to pick a region of DNA from a very small sample and amplify it to some usable quantity. It works by iterating cycles in which only the region of interest is amplified.
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PCR techniques are used to produce sufficient quantities of DNA for this technique.
Southern Blotting ...
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PCR - see polymerase chain reaction.
phage - virus that infects bacteria; altered phage can be used as cloning vectors. (short for bacteriophage - "bacteria eater") ...
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PCR - See POLYMERASE CHAIN REACTION
PEPTIDE - A chain formed by two or more amino acids linked through peptide bonds: dipeptide = two amino acids, oligopeptide = small number of amino acids, etc.
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PCR: see Polymerase Chain Reaction.
Phagemid: A type of plasmid which carries within its sequence a bacteriophage replication origin.
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PCR. See Polymerase chain reaction.
Pedigree. A diagram mapping the genetic history of a par- ticular family.
Persistence. Ability of an organism to remain in a particular setting for a period of time after it is introduced.
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PCR: See polymerase_chain reaction.
Penetrance: Refers to the proportion of individuals heterozygous for a given dominant allele that express the phenotype of that dominant allele.
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GPCRs (G-protein-coupled receptors) play key roles in many cellular processes, and malfunction may lead to a range of pathologies, including psychiatric and neurological disorders.
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Serology and PCR (polymerase chain reaction) studies are available to confirm the diagnosis of dengue if clinically indicated.
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Treatment ...
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Click here for PCR Glossary.
Click here for hundreds of software tools for biomedical studies.
Web ChangBioscience.com ...
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To increase the number of copies of a DNA sequence, in vivo by inserting into a cloning vector that replicates within a host cell, or in vitro by polymerase chain reaction (PCR). Anaerobe. An organism that grows in the absence of oxygen. See Aerobe.
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PCR (Polymerase Chain Reaction) A repetitive procedure that results in a geometric amplification of a specific DNA sequence.
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To replicate DNA in vitro, PCR takes advantage of a special property of the molecule: the hydrogen bonds. These bonds, which bind the complementary strands of DNA together in a double helix, are broken at elevated temperatures (about 95 C).
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polymerase chain reaction (PCR) A method of amplifying or copying DNA fragments that is faster than cloning. The fragments are combined with DNA polymerase, nucleotides, and other components to form a mixture in which the DNA is cyclically amplified.
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polymerase chain reaction (PCR) - technique for amplifying specific regions of DNA by multiple cycles of DNA polymerization, each followed by a brief heat treatment to separate complementary strands.
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(cDNA): Single-stranded DNA produced from an RNA template (usually mRNA) by
reverse transcriptase
in vitro. It lacks the introns present in corresponding genomic DNA. It is most commonly made to use in PCR to amplify RNA (
RT
-PCR).
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PCR -- polymerase chain reaction; a technique for copying the complementary strands of a target DNA molecule simultaneously for a series of cycles until the desired amount is obtained.
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