Overhang: A terminus of a duplex
DNA molecule which has one or more unpaired
nucleotides in one of the two
strands (hence either a 3' or 5'
overhang).
Cleavage of
DNA with many
restriction endonucleases leaves such
overhangs (see
Sticky End).
Full article >>>The
overhangs thus produced can still
hybridize ("
anneal") with each other, even if they came from different parent
DNA molecules, and the
enzyme ligase will then covalently link the
strands.
Full article >>>Homopolymer tailing Extension of the 3' ends of a piece of double-
stranded
DNA using the
enzyme terminal
transferase and a single deoxy
nucleoside triphosphate as
substrate, resulting in a 3'
overhang composed of a single
base repeat.
Full article >>>The fragment ends are then blunted by treatment with Mung Bean
nuclease and T4
DNA polymerase, and are ligated to the blunt-end side of an
adapter which has a 3'
overhang (ACAC) at the other end.
Full article >>>Because recognition
sequences differ between
restriction enzymes, the length and the exact
sequence of a sticky-end "
overhang", as well as whether it is the 5' or the 3'
strand that
overhangs, depends on which
enzyme produced it.
Full article >>>Overhang PAGE See
Polyacrylamide Gel Electrophoresis (
PAGE)
PCR See
Polymerase chain re
action (
PCR)
PFGE See Pulsed-Field
Gel Electrophoresis (
PFGE)
Phenotype Phosphatase Polyacrylamide Gel Electrophoresis (
PAGE)
Polymerase Chain Re
action (
PCR) ...
Full article >>>The ends of the cut have an
overhanging piece of single-
stranded
DNA.
These are called "
sticky ends" because they are able to
base pair with any
DNA molecule containing the
complementary
sticky end.
Full article >>>: Also known as
sticky end.
Overhanging ends of
a double-
stranded
DNA molecule that are
capable of
hybridizing with
complementary ends.
Complementary (copy)
DNA ...
Full article >>>The end of a t
RNA molecule to which an
amino acid becomes bound. It contains both the 5' and 3' ends of the t
RNA. The 3'-terminal
sequence of cytidine-cytidine-
adenosine (CCA)
overhangs the end, ...
Full article >>>cohesive ends Two
DNA ends in the same
DNA molecule, or in different
molecules, with short
overhanging single-
stranded
segments that are
complementary to one another, facilitating
ligation of the ends; also known as
sticky ends.
Full article >>>'"/>