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Origin of replication


Origin of replication
Discrete positions on a chromosome or vector DNA where replications are initiated. Vectors must have at least one origin of replication sequence for their replication in host cells.
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Origin of replication (ori)
The nucleotide sequence where DNA replication is initiated. Determines the specific position on a DNA molecule where DNA replication begins.
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origin of replication
A specific sequence of bases in a nucleic acid molecule to which the enzymes responsible for replicating the nucleic acid bind to initiate the copying process.
osmoconformer ...
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Origin of replication. The nucleotide sequence at which DNA synthesis is initiated.
OSHA. See Occupational Safety and Health Administration.
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Origin of replication: Nucleotide sequences present in a plasmid which are necessary for that plasmid to replicate in the bacterial host. (Abbr. "ori") ...
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Origin of replication: A site at which DNA replication is initiated. There is only one in bacterial chromosomes, but numerous origins in eukaryotic chromosomal DNA. (Abbr. "ori") ...
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Short for origin of replication. The specific nucleotide sequence at which DNA synthesis is initiated. Cf. transcription start site and terminator.
Related Terms: DNA replication ...
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Artificial chromosome A vector constructed from host cell chromosomal elements such as origin of replication, telomeres and centromere (in eukaryotes).
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In the initiation step, several key factors are recruited to an origin of replication. This origin of replication is unwound, and the partially unwound strands form a "replication bubble", with one replication fork on either end.
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This works fine for the 3' -> 5' strand of a chromosome as the DNA polymerase can move uninterruptedly from an origin of replication until it meets another bubble of replication or the end of the chromosome.
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Protein that recognizes the origin of replication on a replicon and takes part in primosome construction.
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: Autonomously replicating sequence.
ARS
is the origin of replication in yeast.
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Every plasmid contains at least one DNA sequence that serves as an origin of replication or ori (a starting point for DNA replication), which enables the plasmid DNA to be duplicated independently from the chromosomal DNA (Fig. 2) ...
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A first-generation episomal vector based on the latent origin of replication oriP and its transactivator EBNA-1 from EBV was used to establish and maintain up to 350 kb of circular DNA in human cells.
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Cloning vectors minimally contain an origin of replication, selectable marker gene (e.g., ampicillin resistance gene), and multiple cloning site containing unique restriction enzyme sites; other useful features may also be present.
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Once the polymerases have opened the molecule, an area known as the replication bubble forms (always initiated at a certain set of nucleotides, the origin of replication).
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Origin of replication. The nucleotide sequence at which DNA synthesis is initiated. OSHA. See Occupational Safety and Health Administration. Overlapping reading frames.
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