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Northern blot


Northern blot
A technique for detecting specific RNA using a labeled DNA probe. RNA (e.g. total polyA RNA) is separated by electrophoresis in agarose gel and transferred onto a membrane.
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Dr. John Carpten, of the National Human Genome Research Institute's Laboratory of Cancer Genetics, defines Northern blot.
related
terms:
cDNA library, deoxyribonucleic acid (DNA), hybridization, mRNA ...
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Northern blotting
Method by which RNA is analyzed. RNA is separated by size, ...
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Northern blot A gel-based laboratory procedure that locates mRNA sequences on a gel that are complementary to a piece of DNA used as a probe.
See also: DNA, library ...
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Northern blotting
Main article: Northern blot
The Northern blot is used to study the expression patterns a specific type of RNA molecule as relative comparison among of a set of different samples of RNA.
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NORTHERN BLOT - RNA molecules are separated by electrophoresis (usually in an agarose gel) on the basis of size, ...
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Northern blot: A technique for analyzing mixtures of RNA by transfer of size-separated RNA fragments to a synthetic membrane, whereby the presence and rough size of one particular type of RNA (usually an mRNA) can be ascertained.
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Northern blotting. See Northern hybridization. Northern hybridization. (Northern blotting). A procedure in which RNA fragments are transferred from an agarose gel to a nitrocellulose filter, where the RNA is then hybridized to a radioactive probe.
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specific DNA or RNA molecules bearing the complementary sequence. A labeled (radioactive; antigen; enzyme; fluorescent) nucleic acid complementary to the sequence being searched for in a restriction digest, genome library, northern blot or in situ ...
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Northern Blot RNA blot taken from an electrophoresis gel.
Nucleic acid A polymeric molecule composed of nucleotide monomers joined by phosphodiester bonds.
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[diagram] (2) of Ranvier noggin gene Nondisjunction Nonhomologous End-Joining (NHEJ) Nonsense-Mediated mRNA Decay (NMD) Noradrenaline (norepinephrine) Northern blot NO synthases (NOS) Notochord[in frog embryology] N-regions of antigen receptor genes ...
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