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Nick translation
A method which uses DNA polymerase I to first produce a nick in a DNA duplex, then degrade stretch of single-stranded DNA using its 5'-exonuclease while synthesizing a new strand in its place.
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Nick translation
Method used to introduce 32p into a DNA probe so that the probe can be detected.
Related Terms: Deoxyribonucleic acid (DNA) ...
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Nick K. Yankovsky, B. I. Kapanadze, V. M. Brodjansky, and G. E. Sulimora
Laboratory of Genome Analysis; Institute of General Genetics; Russian Academy of Sciences; Moscow 117809, Russia
+7-095/135-4307, Fax: -1289, Internet: bion@glas.apc.org ...
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NICK TRANSLATION - A method for introducing labeled nucleotides into a double-stranded DNA molecule which involves making small nicks in one strand with DNase, and then repairing with DNA polymerase I.
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Nick translation: A method for incorporating radioactive isotopes (typically 32P) into a piece of DNA. The DNA is randomly nicked by DNase I, and then starting from those nicks DNA polymerase I digests and then replaces a stretch of DNA.
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Nick translation. A procedure for making a DNA probe in which a DNA fragment is treated with DNase to produce single-stranded nicks, followed by incorporation of radioactive nucleotides from the nicked sites by DNA polymerase I.
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49-58 6 The relationship between higher-order chromatin structure and transcription Nick Gilbert and Wendy A. Bickmore..........
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The first structure postulated in the model (1) has a nick in one strand of one duplex and pairing of one end of the nick with the complementary strand of the other duplex.
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If the F-plasmid is free, a nick is produced in one of the circular DNA strands of the plasmid. Then, the single-stranded DNA of the nicked strand is inserted into the recipiane bacterium (5'-end first).
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A recombinase creates a nick in one strand of a DNA double helix, allowing the nicked strand to pull apart from its complementary strand and anneal to one strand of the double helix on the opposite chromatid.
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A website associated with al-Qaeda posted a video of a man named Nick Berg being decapitated in Iraq. Other decapitation videos and pictures, namely that of Paul Johnson and Kim Sun-il, were first posted onto internet websites.
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