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Minicircles


Minicircles are small (~4kb) circular plasmid derivatives that have been freed from all prokaryotic vector parts.
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... into a number of plasmid-like minicircles, mostly containing one or more genes, ... We show that 'empty' minicircles are also transcribed. ...
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The free minicircles then undergo replication, ultimately forming singly and ... The partially or fully replicated minicircles migrate to two antipodal sites ...
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The EMBO Journal is a science journal containing review articles and research papers in the broad areas of ... Minicircles, however, are unconventional, ...
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Bending modes of DNA directly addressed by cryo-electron microscopy of DNA minicircles ... to probe for the existence of kinks in 94-bp-long minicircles. ...
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Preparation of mitochondrial telomeric minicircles by alkaline lysis ... To relax supertwisted minicircles, 4 µl of DNA was incubated for 60 min at 15°C ...
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Minicircles are released from the network into the kinetoflagellar zone, located ... A low background of linearized minicircles, which are not minicircle replication ...
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When kDNA replication begins, minicircles are individually released from the ... Finally, the progeny minicircles, still containing at least one nick or gap, are ...
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In Cryptobia helicis, these minicircles are supercoiled and non-catenated (13) ... Its minicircles encode classical guide RNA genes (14), which serve for editing ...
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posed of thousands of catenated minicircles ranging in size from ... minicircles encode gRNAs, which are complementary ... Concatenation of minicircles in the ...
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This search yielded 20 minicircles encoding new gRNAs that covered most of the ... Alignments of the minicircles en ... minicircles and subsequent genetic drift. ...
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Kinetoplast DNA minicircles of phloem-restricted Phytomonas ... amplification of Phytomonas minicircles. ... Mitochondrial minicircles contain the genes for guide ...
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Here, the free minicircles initiate unidirectional theta structure replication. ... When all the minicircles have been replicated and reattached, the final gaps are ...
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Replication of minicircles initiates at unique origins that are bound by ... Although many gaps in free minicircles are repaired quickly, some persist even ...
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Leishmania minicircles show features that make them almost ideal as molecular ... 12mer sequence, present in all Leishmania minicircles, is marked in bold. ...
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Kinetoplast DNA minicircles encode guide RNAs for editing of cytochrome oxidase ... The attachment of minicircles to kinetoplast DNA networks during replication ...
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From molecules to physiology ... The progeny free minicircles are thought to migrate to the ... This process continues until all minicircles have replicated. ...
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Comparison of these minicircles with those previously sequenced from H. ... The replication of plastid minicircles involves rolling circle intermediates ...
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