Melting temperature

Melting temperature
At the melting temperature half of the oligo will form duplex with complementary oligo at the same concentration. PCR annealing temperature is usually set a few degrees lower than the melting temperature. See also PCR Glossary.
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The choice of the length of the primers and their melting temperature depends on a number of considerations.
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for the phosphotriester method of DNA synthesis.[3] It has been used to prepare by solution synthesis several trinucleotides that include sequences containing 5MeC and I. The unnatural nucleotides are intended to 1) increase the melting temperature ...
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As this water rises into the mantle of the overriding plate, it lowers its melting temperature, resulting in the formation of magma with large amounts of dissolved gases.
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Since the sequences of the two strands in a heteroduplex differ, the molecule is not perfectly base-paired; the melting temperature of a heteroduplex DNA is dependent upon the number of mismatched base pairs.
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