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Ligase


In biochemistry, a ligase is an enzyme that can catalyse the joining of two large molecules by forming a new chemical bond, usually with accompanying hydrolysis of a small chemical group pendant to one of the larger molecules. Generally ligase catalyses the following...
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In molecular biology, DNA ligase is a special type of ligase (EC 6.5.1.1) that can link together two DNA strands that have double-strand break . The alternative, a single-strand break, is fixed by a different type of DNA...
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DNA ligase This article does not cite any references or sources. ... In addition, DNA ligase has extensive use in ... ATP is required for the ligase reaction. ...
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ligase ( ) n. Any of a class of enzymes that catalyze the linkage of two molecules, generally utilizing ATP as the energy donor
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<I>Taq</I> DNA Ligase catalyzes the formation of a phosphodiester bond between ... Is Taq DNA Ligase used for a special technique? ...
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Catalyzes the formation of a phosphodiester bond between juxtaposed 5' phosphate ... What controls should be run to test the cells and DNA when using T4 DNA Ligase? ...
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... on EC 6.5.1.1 - DNA ligase (ATP) ... poly(deoxyribonucleotide) ligase (AMP-forming) SYNONYMS ... Deoxyribonucleic acid ligase. Deoxyribonucleic acid ...
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Information on EC 6.5.1.2 - DNA ligase (NAD ... Poly(deoxyribonucleotide):poly(deoxyribonucleotide) ligase (AMP-forming, NMN-forming) ...
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... used DNA ligase is derived from the T4 bacteriophage. T4 DNA ligase requires ATP as ... A DNA ligase from E. coli is also available, but is not commonly used. ...
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The T4 DNA Ligase catalyzes the formation of a phosphodiester bond between ... The T4 DNA Ligase binding to DNA may result in a band shift in agarose gels. ...
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The T4 RNA Ligase catalyzes the ATP-dependent intra- and intermolecular ... Uhlenbeck, O.C., Gumport, R.I., T4 RNA ligase, The Enzymes (Boyer, P.D., Ed. ...
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This reaction is usually catalyzed by a DNA ligase enzyme. ... Ligase buffer may be spiked with additional ATP. ... read that ligase can inhibit transformation. ...
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2x Quick ligase buffer (in 40µl aliquots; ... Add 0.5 μL of Quick ligase. ... Also, the ligase, like most enzymes, is in some percentage of glycerol which ...
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ligase - Biological definition of ligase from A Dictionary of Biology at Encyclopedia.com ... ligase Any of a class of enzymes that catalyse the ... DNA ligase ...
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ligase - Definition of ligase from A Dictionary of Plant Sciences at Encyclopedia.com ... ligase An enzyme that catalyses a reaction which joins 2 substrates, using ...
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Sedimentation analysis indicates that the ligase and kinase/CPD domains are ... tRNA ligase and T4 Rnl1 are functionally and structurally homologous proteins ...
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The European Bioinformatics Institute ... In human DNA ligase I (P18858), and in Saccharomyces cerevisiae (Baker's yeast) ... DNA ligase, conserved site ...
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EPICENTRE develops and manufactures reagents and kits for genomics, proteomics, ... Thermostable RNA Ligase. rGka DNA Polymerase. RNA 5' Polyphosphatase ...
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USB Corporation is an established supplier of reliable biochemicals and molecular biology products fueling innovation in both basic research and emerging technology ...
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The smallest known ATP-dependent DNA ligase is the one from the bacteriophage T7 ... http://www.biochem.ucl.ac.uk/bsm/xtal/teach/repl/ligase.html ...
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