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Lambda phage


The life cycle of lambda phages is controlled by cI and Cro proteins. The lambda phage will remain in the lysogenic state if cI proteins predominate, but will be transformed into the lytic cycle if cro proteins predominate.
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Lambda phage
Enterobacteria phage λ (lambda phage) is a temperate phage that lives in E. coli. Once the phage is inside its host, it may integrate itself into the host's DNA.
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Small size range samples consist of restriction digests of Lambda phage DNA along with the complete Lambda genome.
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Cosmids replicate as plasmids in the host cell, but are inserted into the bacterium from lambda phage infectious particles, since transformation would be extremely inefficient with plasmids of this size.
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Expression clone: This is a clone (plasmid in a bacteria, or maybe a lambda phage in bacteria) which is designed to produce a protein from the DNA insert.
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Cosmids can be packaged in lambda phageparticles for infection into Escherichia coli; this permits cloning of larger DNA fragments (up to 45 kb) than can be introduced into bacterial hosts in plasmid vectors.
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A hybrid plasmid that contains cos sites at each end. Cos sites are recognized during head filling of lambda phages. Cosmids are useful for cloning large segments of foreign DNA (up to 50 kb).
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Cosmid
A cloning vector consisting of the phage lambda cos site inserted into a plasmid. Such vectors can be packaged into lambda phage or maintained as plasmids. Cosmids are often used to clone large DNA fragments (up to about 40 kilobases).
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The target DNA sequences can be on the same or on different strands.
If on different strands, an insertion, or integration, as occurs during lysogeny by lambda phage, results.
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An E. Coli virus. Commonly used bacteriophases in biology labs are T4, M13 and lambda phages.
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