In situ hybridization

in situ hybridization
locating a gene by adding specific radioactive probes for the gene and detecting the location of the radioactivity on the chromosome after hybridization ...
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Thomas Ried defines fluorescence in situ hybridization (FISH).
illustrated:
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In situ hybridization
Use of a DNA or RNA probe to detect the presence of the complementary DNA sequence in chromosome spreads or in interphase nuclei or an RNA sequence of cloned bacterial or cultured eukaryotic cells.
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In situ hybridization Use of a DNA or RNA probe to detect the presence of the complementary DNA sequence in cloned bacterial or cultured eukaryotic cells.
In vitro Studies performed outside a living organism such as in a laboratory.
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In situ hybridization
A technique for gene mapping involving hybridization of a labelled sample of a cloned gene to a large DNA molecule (usually a chromosome), often within a cell.
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in situ hybridization - detection of the location of nucleic acid sequences in a cell or organism.
inducer gene - gene encoding the repressor protein of the lac operon; when lactose binds the repressor protein, the lac operon is induced.
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In situ hybridization: Use of a DNA or RNA probe to detect the presence of the complementary DNA sequence in cloned bacterial or cultured eukaryotic cells.
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FISH (fluorescence in situ hybridization): A mapping technique that uses fluorescent tags to identify specific locations of chromosomes.
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FISH -- florescent in situ hybridization: a technique for uniquely identifying whole chromosomes or parts of chromosomes using florescent tagged DNA.
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In most NFT-bearing neurons, we observed a strong reduction in acetylated a-tubulin immunoreactivity (a marker of stable microtubules) and a reduction of the in situ hybridization signal for tubulin mRNA.
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This micrograph uses fluorescence in situ hybridization (FISH) to reveal the ABL DNA (red) and the BCR DNA (green) in the interphase nuclei of the leukemic cells of a patient with CML.
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One of those is called "in situ hybridization." One thing that I would've loved to have known back when I was initially studying the antennapedia mutant in fruit flies is where certain genes are expressed in the body.
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In this newer technique, several different probes specific of one chromosome pair, carrying different amounts of a set of fluorescent dyes, are hybridized to the chromosomes in a technique known as fluorescent in situ hybridization (FISH).
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The high local DNA concentration of aligned DNA sequences make polytene chromosomes ideal targets for in situ hybridization with specific sequence probes.
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or RNA molecules bearing the complementary sequence. A labeled (radioactive; antigen; enzyme; fluorescent) nucleic acid complementary to the sequence being searched for in a restriction digest, genome library, northern blot or in situ hybridization.
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The distribution of RNA transcripts in tissue sections by the use of radiolabeled, complementary oligonucleotides or ribonucleic acids ("riboprobes") is called in situ hybridization histochemistry.
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