Hybrid

Hybridisation between two closely related species is actually a common occurrence in nature. Many hybrid zones are known where the ranges of two species meet, and hybrids are continually produced in great numbers.
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hybridoma
a hybrid cell made from the fusion of a cancer cell with a lymphocyte that has been induced to produce antibodies
Source: Jenkins, John B. 1990. Human Genetics, 2nd Edition. New York: Harper & Row ...
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Definition of trihybrid :
An organism heterozygous at three loci.
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(1) To form a hybrid by performing a cross.
(2) To anneal nucleic acid strands from different sources.
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z ...
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In genetics, hybridisation is the process of mixing different species or varieties of organisms.
In molecular biology hybridisation is the process of joining two complementary strands of DNA.
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Written in 1865 by Gregor Mendel, Experiments on Plant Hybridization was the result after years spent studying genetic traits in pea plants.
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Hybridization conditions: higher the stringency, lower the probability of hybridization. Increase the temperature or decreasing the salt concentration raises the stringency.
Related
Hybridization Melting temperature ...
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Hybridization
The process of joining two complementary strands of DNA or one each of DNA and RNA to form a double-stranded molecule. One strand is often labeled and used as a probe to detect the presence of the second strand. Cf. hybrid.
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Hybrids. Allen Bale (Yale University) reported the submission of four hybrids containing defined 9q deletions to Coriell.
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Hybridization between different species often yields infertile offspring. [Link to a discussion of this postzygotic isolating mechanism.]s But in plants, this does not necessarily doom the offspring.
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Hybridization
A technique where a denatured (single-stranded) nucleotide chain (DNA or RNA) is allowed to pair with another single-stranded nucleotide chain.
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hybrid
[L. hybrida, the offspring of a tame sow and a wild boar]
(1) Offspring of two parents that differ in one or more inheritable characteristics. (2) Offspring of two different varieties or of two different species.
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HYBRIDOMA - A clone of plasmacytoma cells which secrete a monoclonal antibody; usually produced by fusion of peripheral or splenic plasma cells taken from an immunized mouse with an immortalized murine plasmacytoma cell line (fusion partner), ...
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hybrid Offspring of a cross between two species or between alternate homozygous conditions.
hybridoma Fused product of a normal and a myeloma (cancer) cell, which has some of the characteristics of the normal cell.
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Hybridization. The hydrogen bonding of complementary DNA and/or RNA sequences to form a duplex molecule. (See Northern hybridization, Southern hybridization.) ...
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Hybridization: The reaction by which the pairing of complementary strands of nucleic acid occurs. DNA is usually double-stranded, and when the strands are separated they will re-hybridize under the appropriate conditions.
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A hybrid is a heterozygote. Example: Aa
Meiosis, Gamete Formation
The three diagrams below show metaphase I, anaphase I and telophase I in an "Aa" individual.
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dihybrid cross In genetics, a cross that involves two sets of characteristics. PICTURE
dinoflagellates Single-celled to colonial protistans characterized by two flagella, one girdling the cell and the other trailing the cell.
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Two-hybrid does the same thing. It takes a particular protein that is a transcription factor which has two different functions.
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in situ hybridization - detection of the location of nucleic acid sequences in a cell or organism.
inducer gene - gene encoding the repressor protein of the lac operon; when lactose binds the repressor protein, the lac operon is induced.
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In the authors' hybrid GA, a shortest-path-first algorithm, which minimizes the number of "hops" a given data packet must pass through, is used to generate the seed for the initial population.
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chromosome, gene, hybridization, mapping
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DNA renaturation (hybridization) - process whereby two complementary nucleic acid strands form a double helix during an annealing period; a powerful technique for detecting specific nucleotide sequences ...
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Cross-hybridization. The hydrogen bonding of a single- stranded DNA sequence that is partially but not entirely complementary to a singlestranded substrate.
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DNA hybridization -- a technique for selectively binding specific segments of single-stranded (ss) DNA or RNA by base pairing to complementary sequences on ssDNA molecules that are trapped on a nitrocellulose filter.
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probe A labeled fragment of nucleic acid containing a nucleotide sequence complementary to a nucleotide sequence that one wishes to detect in a hybridization experiment.
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This is necessary prior to probe hybridisation and most methods involving enzymic DNA synthesis on a DNA template e.g. sequencing, PCR, some labelling methods. Denaturation may be achieved by heating or treatment with NaOH.
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In most NFT-bearing neurons, we observed a strong reduction in acetylated a-tubulin immunoreactivity (a marker of stable microtubules) and a reduction of the in situ hybridization signal for tubulin mRNA.
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The blot was probed by hybridization with a cloned fragment of the tree's genome.
For this particular combination of restriction enzyme and hybridization probe, the pattern of hybridizing bands shown above was obtained.
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fluorescence in situ hybridisation
)
: One of the more modern methods in cytogenetics, which uses fluorescence-labelled chromosome-specific DNA, probes to detect translocations, inversions, deletions, ...
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FISH (fluorescence in situ hybridization): A mapping technique that uses fluorescent tags to identify specific locations of chromosomes.
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reticulation -- Joining of separate lineages on a phylogenetic tree, generally through hybridization or through lateral gene transfer. Fairly common in certain land plant clades; reticulation is thought to be rare among metazoans.
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Definition: The F1 (first filial) generation is the hybrid offspring produced in the cross pollination of P generation plants.
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