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Hfr cell


Hfr cell
A hfr cell (also called hfr strain) is a bacterium with a conjugative plasmid (often F) integrated into its genomic DNA. Hfr is the abbreviation for high frequency recombination.
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As the chromosome of the Hfr cell replicates it may begin to cross the pilus so that plasmid and chromosomal DNA transfers to the recipient cell. Such DNA may recombine with that of its new host, introducing new gene variants.
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F-factor
An E. coli plasmid that encodes conjugative transfer ("fertility") functions. The F-plasmid may exist as an autonomous plasmid in the cytoplasm or may integrate into specific sites in the chromosome, producing an Hfr cell.
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coli genome was originally mapped by interrupted mating experiments, in which various Hfr cells in the process of conjugation were sheared from recipients after less than 100 minutes (initially using a Waring blender) and investigating which genes ...
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