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Heteroduplex analysis


Heteroduplex analysis
The study of the mobilities of heteroduplex DNA under polyacrilamide gel electrophoresis. The reduced mobility of heteroduplex DNA compared with homoduplex DNA is proportional to the degree of divergence of the sequences.
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The mutations are identified by high-throughput heteroduplex analysis followed by DNA sequencing of PCR products from this large bank. Stocks of mice carrying the specific gene mutation can be reconstituted from the frozen gametes.
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heteroduplex analysis: denatured suspected mutant DNA is annealed with denatured reference DNA; mismatches are detected by altered mobility in electrophoresis.
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