Genomic DNA

Genomic DNA
DNA from the genome containing all coding (exon) and noncoding (intron and other) sequences in contrast to cDNA. which contains only coding sequences.
Related Terms: Deoxyribonucleic acid (DNA) ...
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genomic DNA - nuclear DNA of the chromosomes.
genomic equivalence - concept that each cell in the body has the same genetic material and therefore all the information necessary to create a complete organism.
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CFTR Genomic DNA Sequence -This sequence provided by the Cystic Fibrosis Mutation Database includes segments of noncoding DNA and exons broken down into codons and corresponding amino acids. Links to the GenBank record for each exon are also provided.
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Satellite DNA A genomic DNA fraction which has a different density to the main body of genomic DNA and therefore forms a separate band (satellite) during density gradient ultracentrifugation.
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A library composed of fragments of genomic DNA. (See Library.) Genotype. The structure of DNA that determines the expression of a trait. See Phenotype. Genus. A category including closely related species.
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CGH and FISH is a method for analyzing genomic DNA for unbalanced genetic alterations.
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In the first step of AFLP analysis, genomic DNA is digested with both a restriction enzyme that cuts frequently (MseI, 4 bp recognition sequence) and one that cuts less frequently (EcoRI, 6 bp recognition sequence).
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Genomic blot: A type of Southern blot specifically used to analyze a mixture of DNA fragments derived from total genomic DNA.
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A hfr cell (also called hfr strain) is a bacterium with a conjugative plasmid (often F) integrated into its genomic DNA. Hfr is the abbreviation for high frequency recombination.
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genomic DNA) is separated by electrophoresis in agarose gel and transferred onto a membrane. The membrane is then probed with labeled DNA to detect the specific sequence. Named after its inventor, Dr. Southern.
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Genomics
The comparative analysis of genomic DNA sequences from different organisms. Genomic analysis can provide information about the evolution of genes and can make predictions about the metabolism of an organism.
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Genomic library. A library composed of fragments of genomic DNA. (See Library.)
Genotype. The structure of DNA that determines the expression of a trait. See Phenotype.
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plasmid A small circle of DNA that may be carried by a bacterium in addition to its genomic DNA.
plasmodesma A cytoplasmic strand that connects adjacent plant cells through pores in the cell wall.
plasmodium Multinucleate ameboid mass, syncytial.
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Computer programs to find splicing sites in genomic DNA sequences
5
Alternative splicing ...
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complementary DNA (CDNA) - DNA molecule made as a copy of mRNA and therefore lacking the introns that are present in genomic DNA
cristae - folds of the inner mitochondrial membrane ...
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Southern blotting allows the detection of fragments of genomic DNA, which anneal to the radiolabeled probe. The fragments are generated using restriction enzymes and separated in a gel by electrophoresis.
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(cDNA): Single-stranded DNA produced from an RNA template (usually mRNA) by
reverse transcriptase
in vitro. It lacks the introns present in corresponding genomic DNA. It is most commonly made to use in PCR to amplify RNA (
RT
-PCR).
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