Ethidium Bromide

Ethidium bromide
A fluorescent chemical that intercalates between base pairs in a doublestranded DNA molecule. Commonly used to detect DNA following gel electrophoresis. Ethidium bromide intercalates between the stacked bases of DNA and RNA.
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Ethidium Bromide: Intercalates within the structure of nucleic acids in such a way that they fluoresce under UV light. Ethidium bromide staining is commonly used to visualize RNA or DNA in agarose gels placed on UV light boxes.
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Ethidium bromide. A fluorescent dye used to stain DNA and RNA. The dye fluoresces when exposed to UV light. Eukaryote.
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reaction insertion mutation A mutation caused by insertion of one or more extra bases between bases in DNA, or a mutagen which itself is inserted between bases (like ethidium bromide).
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Digests are analyzed on standard agarose gels, which are post-stained with ethidium bromide or thiazole orange and imaged with a Molecular Dynamics FluorImager 575.
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Ethidium bromide, Silver, or Coomassie blue dye can be used. Other methods can also be used to visualize the separation of the mixture's components on the gel.
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The DNA bands must be stained to make them visible. Ethidium bromide-stained DNA will fluoresce when illuminated with UV light.
PCR techniques are used to produce sufficient quantities of DNA for this technique.
Southern Blotting ...
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Gamma and Alpha Radiation, ionising radiations
Transposons, autonomous dna fragment relocation/multiplication
Base analogues, substitutes
Bromine and some of its compounds,
Ethidium bromide (EtBr), Intercalating agents.
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fragments at four different discrete sizes, those four size classes will, when subjected to electrophoresis, all migrate in groups, producing four migrating "bands". Usually, these are visualized by soaking the gel in a dye (ethidium bromide) which ...
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