DNA polymerase

DNA polymerase cannot initiate a new strand, it can only elongate a strand that is already present. Synthesis of new DNA therefore cannot begin until a short strand of nucleotides is added. This short strand is called a primer.
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DNA polymerases play an essential role in current methods of DNA sequencing, ...
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DNA polymerase can only synthesize a new strand of DNA as it moves along the template strand in the 3' -> 5' direction.
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DNA polymerase occurs naturally in living organisms, where it functions to duplicate DNA when cells divide. It works by binding to a single DNA strand and creating the complementary strand.
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DNA polymerase
Enzymes that catalyze the synthesis of nucleic acids on preexisting nucleic acid templates, assembling DNA from deoxyribonucleotides. Cf. RNA polymerase.
Primer ...
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DNA polymerase - catalyzes the addition, of a deoxyribonucleotide to the 3' end of a DNA chain.
DNA transcription - process in which molecules of RNA are synthesized in which a strand of DNA acts as a template.
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DNA polymerases
Enzymes that polymerize deoxyribonucleotides onto an existing polynucleotide chain using the complementary strand of DNA as a template.
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DNA polymerase In DNA replication, the enzyme that links the complementary nucleotides together to form the newly synthesized strand.
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DNA polymerase
An enzyme that catalyzes the elongation of new DNA at a replication fork by the addition of nucleotides to the existing chain.
DNA probe ...
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DNA polymerase: the enzyme that joins all the nucleotide components to one another to form a long strand of nucleotides.
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DNA POLYMERASE - A polymerase which synthesizes DNA (see POLYMERASE).
DNase - see DEOXYRIBONUCLEASE.
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DNA polymerase occurs naturally in living organisms, where it functions to duplicate DNA when cells divide in mitosis and meiosis. Polymerase works by binding to a single DNA strand and creating the complementary strand.
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DNA polymerase: Enzymatic activity responsible for catalyzing the polymerization of DNA. Is dependent upon an annealed primer from which to initiate polymerization, and a DNA template from which to copy.
DNA probes: See probe.
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mutation of DNA polymerase that increases the overall mutation rate.
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z ...
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Taq polymerase: A DNA polymerase isolated from the bacterium Thermophilis aquaticus and which is very stable to high temperatures. It is used in PCR procedures and high temperature sequencing.
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DNA polymerase. See Polymerase. DNA polymorphism. One of two or more alternate forms (alleles) of a chromosomal locus that differ in nucleotide sequence or have variable numbers of repeated nucleotide units. (See Allele.) DNA polymerase.
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cDNA -- complementary DNA produced from a RNA template by the action of RNA- dependent DNA polymerase.
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It involves using a thermostable DNA Polymerase such as Taq (Thermus aquaticus) and repeatedly heating to denature strands of DNA, followed by cooling to allow polymerization of new strands.
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At the temperature DNA denatures (strands separate) proteins from most organisms (like DNA polymerases) also come apart. This presents a problem.
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Eukaryotic DNA polymerases participate in chromosomal replication, repair, crossing-over and mitochondrial replication. To initiate replication, DNA polymerases require a priming RNA molecule. They extend the DNA using
deoxyribonucleotide ...
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dideoxynucleotides - chain-terminating precursors of DNA synthesis that block further polymerization when added to the end of the DNA strand by DNA polymerase.
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A Thermus aquaticus thermal resistant DNA polymerase. It is the first thermal resistant DNA polymerase used in PCR.
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Deoxynucleotides (A,T,G, and C) and DNA polymerase are added and, in a process called primer extension, the complementary copy of the ssDNA fragment is built. The result is two double-stranded DNA molecules identical to the original.
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Filling-in In vitro synthesis of DNA (by DNA polymerase) to convert an overhanging end of a double-stranded DNA fragment to a blunt end.
Fusion protein A recombinant protein which is composed of amino acid sequences derived from more than one gene.
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