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Complementary sequences


DNA hybridization -- a technique for selectively binding specific segments of single-stranded (ss) DNA or RNA by base pairing to complementary sequences on ssDNA molecules that are trapped on a nitrocellulose filter.
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Base-pairing between overhangs with complementary sequences enables two fragments to join or "to be spliced," which they tend to do spontaneously in a test tube.
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A region of single-stranded DNA or RNA that can form base pairs between complementary sequences. For example the sequence 5'...GACGTGNNNNNCTGCAC...
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A class of RNA having structures with triplet nucleotide sequences that are complementary sequences to the triplet nucleotide coding sequences of mRNA.
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perhaps by binding to complementary sequences on DNA or
perhaps by binding to the nascent RNA transcript as it is being formed.
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DNA hybridization The formation of hybrid DNA molecules that contain a strand of DNA from two different species. The number of complementary sequences in common in the two strands is an indication of the degree of relatedness of the species.
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Hairpin: A helical (duplex) region formed by base pairing between adjacent (inverted) complementary sequences within a single strand of RNA or DNA.
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A single-stranded DNA that has been radioactively labeled and is used to identify complementary sequences in genes or DNA fragments of interest. (See Multilocus probe.) Promoter.
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