Cloning

Cloning of the original DNA in a vector to other vectors. The original DNA may be digested to create smaller subclones.
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Cloning - A collection of resources from MEDLINEplus, a service of the U.S. National Library of Medicine and the National Institutes of Health.
Cloning In Focus - An excellent introduction to cloning from the Genetic Science Learning Center.
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Cloning of any DNA sequence comprises of the following four steps: amplification, ligation, transfection, and screening/selection. Initially, the DNA fragment of interest needs to be amplified (many copies need to be produced).
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Definition of cloning : ...
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This process, used by researchers in the Human Genome Project, is referred to as cloning DNA. The resulting cloned (copied) collections of DNA molecules are called clone libraries.
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Cloning is the process of creating an identical copy of an original. A clone in the biological sense, therefore, is a single cell (like bacteria, lymphocytes etc.) or multi-cellular organism that is genetically identical to another living organism.
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chromosome, cloning, gene, genetic marker, Human Genome Project
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Cloning Animals
Asexual reproduction in bacteria and plants allows scientists to obtain genetically identical populations; this does not occur naturally in vertebrates, except in twins.
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Cloning vector
DNA molecule originating from a virus, a plasmid, or the cell of a higher organism into which another DNA fragment of appropriate size can be integrated without loss of the vectors capacity for self-replication; ...
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Cloning mammals using somatic cell nuclei
Stem cells are cells that divide by mitosis to form either
two stem cells, thus increasing the size of the stem cell "pool",
or ...
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Cloning: The process of asexually producing a group of cells (clones), all genetically identical, from a single ancestor.
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Cloning vector
A DNA molecule that is capable of replication in a suitable host cell, that has suitable site(s) for the insertion of DNA fragments by recombinant DNA techniques, ...
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cloning vector - intentionally designed artificial DNA construct used by molecular biologists to amplify selected pieces of DNA inserted into the construct; examples include plasmid, phage, phagemid, cosmid, fosmid, ...
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cloning vector
An agent used to transfer DNA in genetic engineering, such as a plasmid that moves recombinant DNA from a test tube back into a cell, or a virus that transfers recombinant DNA by infection.
closed circulatory system ...
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Cloning: to allow it to be sequenced or studied in some other way.
Cloning: The process of generating sufficient copies of a particular piece of DNA or asexually producing a group of cells (clones), all genetically identical, from a single ancestor.
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Cloning Mammals
Cloning animals refers to producing offspring that are genetically identical to the animal being cloned.
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Subcloning. The process of tranferring a cloned DNA fragment from one vector to another. (See Cloning.)
Subunit vaccine. A vaccine composed of a purified antigenic determinant that is separated from the virulent organism. (See Vaccine, Enzyme.) ...
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Sub-cloning: If you have a cloned piece of DNA (say, inserted into a plasmid) and you need unlimited copies of only a part of it, you might "sub-clone" it.
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SHOTGUN CLONING or SEQUENCING - Cloning of an entire genome or large piece of DNA in the form of randomly generated small fragments. The individual sequences obtained from the clones will be used to construct contigs.
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Molecular Cloning: A Laboratory Manual (3-Volume Set)
Joseph Sambrook; Paperback; Buy New: $205.00
39.
The Coming Plague: Newly Emerging Diseases in a World Out of Balance
Laurie Garrett; Paperback; Buy New: $11.17 ...
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To increase the number of copies of a DNA sequence, in vivo by inserting into a cloning vector that replicates within a host cell, or in vitro by polymerase chain reaction (PCR). Anaerobe. An organism that grows in the absence of oxygen. See Aerobe.
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Cloned DNA -- any DNA fragment that passively replicates in the host organism after it has been joined to a cloning vector. Codon -- a sequence of three nucleotides in mRNA that specifies an amino acid. Consanguinity -- genetic relationship.
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cDNA (complementary DNA) A DNA, usually made by reverse transcriptase, which is complementary to given mRNA and used in cloning. central dogma The organizing principle of molecular biology: genetic information flows from DNA to RNA to protein.
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Cloning is the term used to describe the production and isolation of large numbers of copies of a specific DNA sequence using a mixture of sequences as a starting point.
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Recent research has capitalized on the cloning of an important signalling receptor for collagen, glycoprotein VI, which is expressed only on platelets, ...
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These additional sequences were isolated by molecular cloning and characterized by sequencing .
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"Cloning and Characterization of a Potassium-dependent Sodium/Calcium Exchanger in Drosophila". J. Cell Biol. 147(3):659-669.
P. Raghu, et al. (2000). "Normal Phototransduction in Drosophila Photoreceptors Lacking an InsP3 Receptor Gene". Molec.
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natality The production of new individuals by birth, hatching, germination, or cloning.
native species A local species that has not been introduced.
natural history The study of where and how organisms carry out their life cycles.
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polymerase chain reaction (PCR) A method of amplifying or copying DNA fragments that is faster than cloning. The fragments are combined with DNA polymerase, nucleotides, and other components to form a mixture in which the DNA is cyclically amplified.
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