Clones

Constructing Clones for Sequencing.
Cloned DNA molecules must be made progressively smaller and the fragments subcloned into new vectors to obtain fragments small enough for use with current sequencing technology.
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clones doubly mutant for ptc and ci exhibit the same wing defects (see mutations) as ci single mutant clones. (Methot, 2001) ...
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Clones containing recombinant DNA molecules. See recombinant DNA technologies.
Related Terms: Clone bank
See genomic library.
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Clones: A group of cells derived from a single ancestor.
Clone (verb):
Clone (verb):. The act of duplicating genetic material within a vector.
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cDNA clones are PCR amplified and the PCR products are printed on to slides with micropins. The clones are verified by sequencing. cDNA array is hybridized with Cy-dye labeled sample and identical control for the same set of experiments.
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Natural clones
Cloning exists in nature in some species and is referred to as parthenogenesis.
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All those clones that continue to grow on ampicillin but fail to grow on kanamycin (here, clones 2, 5, and 8) have been transformed with a piece of human DNA.
Some recombinant DNA products being used in human therapy ...
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genomic 60% cDNA clones 40% cDNA clones
From cells infected with the Sendai paramyxovirus, 40% of cDNA clones recovered had an extra G residue.
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Climate: Tropical cyclones (hurricanes) develop anywhere from off the coast of Africa near Cape Verde to the Windward Islands and move westward into the Caribbean Sea or up the east coast of North America; hurricanes can occur from May to December, ...
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Working from a flanking DNA marker, overlapping clones are successively identified that span a chromosomal region of interest. (See Chromosome.) Cistron. A DNA sequence that codes for a specific polypeptide; a gene. See DNA, Gene. Clone.
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Library A collection of clones or DNA fragments which contains all of the sequences present in the source.
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clones The descendants of a single cell. Classically, this has meant cells, but Dolly the sheep was a clone.
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Histochemical, immunohistochemical and functional analysis of the resulting cybrids all showed a pattern in the PD clones indicative of a mtDNA mutation.
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Many horticultural varieties of plants are clones, having been derived from a single individual, multiplied by some process other than sexual reproduction.
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By repeating this process with the thousands of clones that span each chromosome, researchers can determine the sequences of all the larger clones.
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You screen a genomic library for all clones hybridizing with the probe, and then figure out which one extends furthest into the surrounding DNA.
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CHROMOSOME WALKING - The sequential isolation of clones carrying overlapping sequences of DNA which span large regions of a chromosome. Overlapping regions of clones can be identified by hybridization.
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library. In molecular biology, a set of clones containing recombinant DNA. Obtained from and representing the genome of the organism.
lichen An organism composed of a symbiotic association of an ascomycete fungus with algal or cyanobacterial cells.
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Chromosome walking. Working from a flanking DNA marker, overlapping clones are successively identified that span a chromosomal region of interest. (See Chromosome.)
Cistron. A DNA sequence that codes for a specific polypeptide; a gene. See DNA, Gene.
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Genomic library
A collection of clones that includes essentially all the genes of a particular organism.
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Cloning: The process of asexually producing a group of cells (clones), all genetically identical, from a single ancestor.
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Offspring have some genes from each of two different parents and therefore are not identical clones of their parents.
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clone -- An identical copy of an organism. Most plants, fungi, algae, and many other organisms naturally reproduce by making clones of themselves as a form of asexual reproduction.
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Since bacteria reproduce asexually all of its descendents are clones. Since reproduction is not perfect mutations happen. The experimenter can set the environment so that mutations for a particular attribute are selected.
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Contig maps are important because they provide the ability to study a complete, and often large segment of the genome by examining a series of overlapping clones which then provide an unbroken succession of information about that region.
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recombinant DNA technology A series of techniques in which DNA fragments are linked to self-replicating forms of DNA to create recombinant DNA molecules. These molecules in turn are replicated in a host cell to create clones of the inserted segments.
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