Constructing
Clones for
Sequencing.
Cloned
DNA molecules must be made progressively smaller and the fragments subcloned into new
vectors to obtain fragments small enough for use with current
sequencing technology.
Full article >>>clones doubly mutant for ptc and ci exhibit the same wing defects (see
mutations) as ci single mutant
clones. (Methot, 2001) ...
Full article >>>Clones containing
recombinant DNA molecules. See
recombinant DNA technologies.
Related Terms:
Clone bankSee
genomic library.
Full article >>>Clones: A group of
cells derived from a single ancestor.
Clone (verb):
Clone (verb):. The act of duplicating
genetic material within a
vector.
Full article >>>cDNA clones are
PCR amplified and the
PCR products are printed on to slides with micropins. The
clones are verified by
sequencing.
cDNA array is
hybridized with Cy-dye labeled sample and identical control for the same set of experiments.
Full article >>>Natural
clonesCloning exists in nature in some
species and is referred to as
parthenogenesis.
Full article >>>All those
clones that continue to grow on ampicillin but fail to grow on kanamycin (here,
clones 2, 5, and 8) have been
transformed with a piece of
human DNA.
Some
recombinant DNA products being used in
human therapy ...
Full article >>>genomic 60%
cDNA clones 40%
cDNA clones
From
cells infected with the Sendai paramyxo
virus, 40% of
cDNA clones recovered had an extra G
residue.
Full article >>>Climate: Tropical cy
clones (hurricanes) develop anywhere from off the coast of Africa near Cape Verde to the Windward Islands and move westward into the Caribbean Sea or up the east coast of North America; hurricanes can occur from May to December, ...
Full article >>>Working from a flanking
DNA marker,
overlapping clones are successively identified that span a chromosomal region of interest. (See
Chromosome.)
Cistron. A
DNA sequence that codes for a specific
polypeptide; a
gene. See
DNA,
Gene.
Clone.
Full article >>>Library A collection of
clones or
DNA fragments which contains all of the
sequences present in the source.
Full article >>>clones The descendants of a single
cell.
Classically, this has meant
cells, but Dolly the sheep was a
clone.
Full article >>>Histochemical, immunohistochemical and functional ana
lysis of the resulting cybrids all showed a pattern in the PD
clones indicative of a
mtDNA mutation.
Full article >>>Many horticultural varieties of
plants are
clones, having been derived from a single individual, multiplied by some process other than
sexual reproduction.
Full article >>>By repeating this process with the thousands of
clones that span each
chromosome, researchers can determine the
sequences of all the larger
clones.
Full article >>>You screen a
genomic library for all
clones hybridizing with the
probe, and then figure out which one extends furthest into the surrounding
DNA.
Full article >>>CHROMOSOME WALKING - The sequential isolation of
clones carrying overlapping
sequences of
DNA which span large regions of a
chromosome. Overlapping regions of
clones can be identified by
hybridization.
Full article >>>library. In
molecular biology, a set of
clones containing
recombinant DNA. Obtained from and representing the
genome of the
organism.
lichen An
organism composed of a symbiotic association of an ascomycete
fungus with algal or
cyanobacterial
cells.
Full article >>>Chromosome walking. Working from a flanking
DNA marker,
overlapping clones are successively identified that span a chromosomal region of interest. (See
Chromosome.)
Cistron. A
DNA sequence that codes for a specific
polypeptide; a
gene. See
DNA,
Gene.
Full article >>>Genomic library A collection of
clones that includes essentially all the genes of a particular
organism.
Full article >>>Cloning: The process of
asexually producing a group of
cells (
clones), all genetically identical, from a single ancestor.
Full article >>>Offspring have some genes from each of two different parents and therefore are not identical
clones of their parents.
Full article >>>clone -- An identical copy of an
organism. Most
plants,
fungi,
algae, and many other
organisms naturally reproduce by making
clones of themselves as a form of
asexual reproduction.
Full article >>>Since
bacteria reproduce
asexually all of its descendents are
clones. Since
reproduction is not perfect
mutations happen. The experimenter can set the
environment so that
mutations for a particular attribute are selected.
Full article >>>Contig maps are important because they provide the ability to study a complete, and often large
segment of the
genome by examining a series of
overlapping clones which then provide an unbroken succession of information about that region.
Full article >>>recombinant DNA technology A series of techniques in which
DNA fragments are linked to self-replicating forms of
DNA to create
recombinant DNA molecules. These
molecules in turn are replicated in a
host cell to create
clones of the
inserted
segments.
Full article >>>'"/>