Chromosome walking

Chromosome walking is a technique to clone a gene (e.g., a disease gene) from its known closest markers. The closest linked marker (e.g., EST or a known gene) to the gene is used to probe a genomic library.
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Chromosome walking is a method in genetics for identifying and sequencing long parts of a DNA strand, e.g., a chromosome.
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Chromosome walking
Method involving hybridizing a primer of known sequence to a clone from an unordered genomic library and synthesizing a short complementary strand (called walking along a chromosome).
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Chromosome walking
A technique used to identify a series of overlapping restriction fragments, often to determine the relative positions of genes on large chromosomes.
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CHROMOSOME WALKING - The sequential isolation of clones carrying overlapping sequences of DNA which span large regions of a chromosome. Overlapping regions of clones can be identified by hybridization.
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Chromosome walking. Working from a flanking DNA marker, overlapping clones are successively identified that span a chromosomal region of interest. (See Chromosome.)
Cistron. A DNA sequence that codes for a specific polypeptide; a gene. See DNA, Gene.
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Chromosome walking: A technique for cloning everything in the genome around a known piece of DNA (the starting probe).
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(See Chromosome walking.) Chromosome walking. Working from a flanking DNA marker, overlapping clones are successively identified that span a chromosomal region of interest. (See Chromosome.) Cistron.
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5000 BACs from a well-represented library where 5 to 10% of the bacterial community is composed of an "archetypal species" (a single species or a closely related group of species) could produce a contig of 1-2 Mbp before chromosome walking fails.
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