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Chromosome walking


Primer walking is a sequencing method of choice for sequencing DNA fragments between 1.3 and 7 kilobases. Such fragments are too long to be sequenced in a single sequence read using the chain termination method. This method works by dividing the long sequence into several consecutive short ones. The DNA of interest may be a plasmid insert, a PCR product or a...
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The article makes a basic mistake, I think: If you are chromosome walking, you are not necessarily sequencing. e.g. You might be mapping. And while you might walk by sequencing minimally the ends of each fragments, I think you could walk just by detecting hybridization and without ever learning the sequence of the overlapping regions. I suspect the technique...
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Encyclopedia information on Chromosome Walking ... Chromosome walking is a technique for cloning everything in the genome around a ...
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Primer walking This article needs additional citations for verification. ... The term "chromosome walking" is used instead when we know the sequence but ...
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Chromosome walking. Chromosome walking. Chromosome walking is a technique to ... several times to walk across the chromosome and reach the gene of interest. ...
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1: Pediatr Res. 2004 Apr;55(4):539-40. Epub 2004 Mar 3. Dead chromosome walking. Vilain E. ... Repetitive Sequences, Nucleic Acid. Y Chromosome*/genetics ...
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Chromosome,walking,biological,biology dictionary,biology terminology,biology terms,biology abbreviations ... CHROMOSOME WALKING - The sequential isolation of ...
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Schematic outline of SEFA PCR method for chromosome walking. ... Chromosome walking of bacterial and fungal genomic DNA. ... Chromosome walking of five plant genomes. ...
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Chromosome walking is a method in genetics for identifying and sequencing long ... The method can be used to sequence entire chromosomes (thus, chromosome walking) ...
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Prev Term: chromosome translocation. Next Term: chronic brain damage. chromosome walking ... of the chromosome to be spanned; often performed to reach a ...
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Chromosome walking ... complementary strand (called walking along a chromosome) ... Chromosome walking is also used to locate specific genes by sequencing the ...
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Figure 1 Schematic outline of SiteFinding-PCR method for chromosome walking. ... Figure 3 Chromosome walking for the Cyanophage P4 and an Arabidopsis mutant ...
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A Versatile Method for Accelerated Chromosome Walking and DNA Fingerprinting. Case Number: 1882 ... Besides direct genome walking, other uses of UFW include ...
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... 1 cM of a disease gene, chromosome walking can be used to clone the disease gene itself. ... to walk across the chromosome and reach the flanking marker ...
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Chromosome walking from un-10 toward wc-1 and oli. ... This chromosome walking in the CBM1 library, together with that done by McClung ...
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Therefore, chromosome walking can be used to clone any gene which can be genetically identified. ... While chromosome walking is technically straight forward, ...
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WHAT IS IT? ... Page 1 of 1: CHROMOSOME WALKING ... Chromosome walking is a procedure to find and sequence a gene whose approximate ...
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