Chromosome walk

Chromosome walking is a technique to clone a gene (e.g., a disease gene) from its known closest markers. The closest linked marker (e.g., EST or a known gene) to the gene is used to probe a genomic library.
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Chromosome walking is a method in genetics for identifying and sequencing long parts of a DNA strand, e.g., a chromosome.
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Chromosome walking
Method involving hybridizing a primer of known sequence to a clone from an unordered genomic library and synthesizing a short complementary strand (called walking along a chromosome).
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Chromosome walking
A technique used to identify a series of overlapping restriction fragments, often to determine the relative positions of genes on large chromosomes.
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CHROMOSOME WALKING - The sequential isolation of clones carrying overlapping sequences of DNA which span large regions of a chromosome. Overlapping regions of clones can be identified by hybridization.
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Chromosome walking. Working from a flanking DNA marker, overlapping clones are successively identified that span a chromosomal region of interest. (See Chromosome.)
Cistron. A DNA sequence that codes for a specific polypeptide; a gene. See DNA, Gene.
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Chromosome walking: A technique for cloning everything in the genome around a known piece of DNA (the starting probe).
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(See Chromosome walking.) Chromosome walking. Working from a flanking DNA marker, overlapping clones are successively identified that span a chromosomal region of interest. (See Chromosome.) Cistron.
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5000 BACs from a well-represented library where 5 to 10% of the bacterial community is composed of an "archetypal species" (a single species or a closely related group of species) could produce a contig of 1-2 Mbp before chromosome walking fails.
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Scoring individuals (or groups of individuals) for the linked RAPD marker should speed the breeding process.
RAPD markers linked to genes of interest can serve as starting points for chromosome walks to isolate those genes.
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