CDNA clone

cDNA clones are PCR amplified and the PCR products are printed on to slides with micropins. The clones are verified by sequencing. cDNA array is hybridized with Cy-dye labeled sample and identical control for the same set of experiments.
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cDNA clone: "complementary DNA"; a piece of DNA copied from an mRNA. The term "clone" indicates that this cDNA has been spliced into a plasmid or other vector in order to propagate it.
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genomic 60% cDNA clones 40% cDNA clones
From cells infected with the Sendai paramyxovirus, 40% of cDNA clones recovered had an extra G residue.
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From the IMAGE cDNA clones, researchers at the Washington University (St.Louis) Sequencing Center determine ESTs with support from Merck, Inc. The data, which are used in gene localization, are then entered into public databases.
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sequencing several hundred base pairs from both ends of cDNA clones taken from a cDNA library), and the resulting sequence is a relatively low quality fragment whose length is limited by current technology to approximately 500 to 800 nucleotides.
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Reverse transcriptase
An enzyme produced by retroviruses that can synthesize a strand of DNA complimentary to an RNA template. (Reverse transcriptase can also synthesize DNA from a DNA template.) Used to make cDNA clones from mRNA.
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