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Avidin


AVIDIN - A glycoprotein which binds to biotin with very high affinity (Kd = 10-15).
BACK MUTATION - Reverse the effect of a point or frame-shift mutation that had altered a gene; thus it restores the wild-type phenotype (see REVERTANT).
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Avidin
A protein that binds biotin with a very high affinity. Used for the detection of biotinylated probes.
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Avidin: A glycoprotein which binds to biotin with very high affinity (Kd = 10-15).
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Biotin has an extraordinarily high affinity for streptavidin with a reported dissociation constant of ~10-15. This very strong binding affinity has made the biotin-streptavidin system very attractive for a multitude of in vitro labeling applications.
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For example, ribonuclease inhibitor binds to ribonuclease A with a roughly 20 fM dissociation constant. Other proteins have evolved to bind specifically to unusual moieties on another protein, e.g., biotin groups (avidin), ...
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