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Affinity chromatography


Affinity Chromatography
The goal of affinity chromatography is to separate all the molecules of a particular specificity from the whole gamut of molecules in a mixture such as a blood serum.
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Affinity chromatography is a method of separating biochemical mixtures, based on a highly specific biologic interaction such as that between antigen and antibody, enzyme and substrate, or receptor and ligand.
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Affinity chromatography is a biochemical separation method that combines size fractionation capability of gel permeation chromatography with the ability to design a stationary phase that reversibly binds to a known subset of molecules.
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Any protein that binds tightly to a specific sugar or sugars. Used in affinity chromatography to purify glycoproteins and as reagents for detecting glycoproteins.
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To characterise protein-protein interactions, a number of chromatography techniques are used especially affinity chromatography.
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